Paradoxical electro-olfactogram responses in TMEM16B knock-out mice.

IF 2.8 4区 心理学 Q1 BEHAVIORAL SCIENCES Chemical Senses Pub Date : 2023-01-01 DOI:10.1093/chemse/bjad003
Giorgia Guarneri, Simone Pifferi, Michele Dibattista, Johannes Reisert, Anna Menini
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Abstract

The Ca2+-activated Cl¯ channel TMEM16B carries up to 90% of the transduction current evoked by odorant stimulation in olfactory sensory neurons and control the number of action potential firing and therefore the length of the train of action potentials. A loss of function approach revealed that TMEM16B is required for olfactory-driven behaviors such as tracking unfamiliar odors. Here, we used the electro-olfactogram (EOG) technique to investigate the contribution of TMEM16B to odorant transduction in the whole olfactory epithelium. Surprisingly, we found that EOG responses from Tmem16b knock out mice have a bigger amplitude compared to those of wild type. Moreover, the kinetics of EOG responses is faster in absence of TMEM16B, while the ability to adapt to repeated stimulation is altered in knock out mice. The larger EOG responses in Tmem16b knock out may be the results of the removal of the clamping and/or shunting action of the Ca2+-activated Cl¯ currents leading to the paradox of having smaller transduction current but larger generator potential.

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TMEM16B基因敲除小鼠的反常电心电图反应。
在嗅觉神经元中,Ca2+激活的Cl¯通道TMEM16B携带了由气味刺激诱发的90%的传导电流,并控制着动作电位点燃的数量,从而控制着动作电位序列的长度。功能缺失法发现,TMEM16B 是嗅觉驱动行为(如追踪陌生气味)所必需的。在这里,我们使用电-olfactogram(EOG)技术研究了 TMEM16B 对整个嗅上皮细胞气味传导的贡献。令人惊讶的是,我们发现与野生型小鼠相比,Tmem16b基因敲除小鼠的EOG反应振幅更大。此外,在 TMEM16B 缺失的情况下,EOG 反应的动力学速度更快,同时基因敲除小鼠对重复刺激的适应能力也发生了改变。Tmem16b基因敲除小鼠更大的EOG反应可能是由于消除了Ca2+激活的Cl'电流的钳位和/或分流作用,从而导致了转导电流变小但发生器电位变大的悖论。
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来源期刊
Chemical Senses
Chemical Senses 医学-行为科学
CiteScore
8.60
自引率
2.90%
发文量
25
审稿时长
1 months
期刊介绍: Chemical Senses publishes original research and review papers on all aspects of chemoreception in both humans and animals. An important part of the journal''s coverage is devoted to techniques and the development and application of new methods for investigating chemoreception and chemosensory structures.
期刊最新文献
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