Early post mortem expression of apoptosis genes in longissimus dorsi muscle of Braford steers differing in managing fed system

Q4 Biochemistry, Genetics and Molecular Biology Animal Gene Pub Date : 2020-06-01 DOI:10.1016/j.angen.2020.200101

M.S. Coria , M.S. Castaño Ledesma , G.A. Palma

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Abstract

The objective of this study was to evaluate the expression of casp9, Bax, Bcl2 Hsp70 and Hsp27 genes in Braford steers differing in finishing strategies. Thirty Braford steers grazing on summer pasture were used for the study. For 120 days fifteen animals were supplemented with corn silage at 1% of body weight per head per day whereas the remaining 15 steers only received pasture. Supplemented steers showed higher WBSF (p = .03) than Control group. Gene expression was measured using real-time polymerase chain reaction. Expression of the casp9, Bax, Bcl2 genes did not differ between groups. Expression of Hsp70 and Hsp27 genes was up-regulated in Suppl group (p < .05). These results suggest a gene by diet interaction in Hsp70 and Hsp27 chaperones and these interaction impact in Warner Bratzler Shear Force in Braford longissimus dorsi muscle.

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不同饲养方式的牛背最长肌死后早期凋亡基因的表达

本研究的目的是评估casp9、Bax、Bcl2、Hsp70和Hsp27基因在不同育肥策略的Braford阉牛中的表达。研究对象为30头在夏季牧场放牧的布拉福德阉牛。在120天的试验中，15头牛每天以每头体重的1%添加玉米青贮饲料，其余15头牛只饲喂牧草。与对照组相比，饲粮添加后的肉牛WBSF显著增加(p = .03)。采用实时聚合酶链反应测定基因表达。casp9、Bax、Bcl2基因的表达在各组间无显著差异。补品组Hsp70和Hsp27基因表达上调(p <. 05)。这些结果表明，Hsp70和Hsp27伴侣基因通过饮食相互作用影响了Braford背最长肌的Warner Bratzler剪切力。

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Animal Gene Agricultural and Biological Sciences-Insect Science

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期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.