In-silico mining and characterization of MYB family genes in wilt-resistant hybrid guava (Psidium guajava × Psidium molle).

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-06-30 DOI:10.1186/s43141-023-00528-3
Israr Ahmad, Sumit K Soni, Muthukumar M, Devendra Pandey
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引用次数: 1

Abstract

Background: The MYB family is one of the most significant groups of transcription factors in plants. However, several MYBs have been linked to secondary metabolism and are important for determining the color of fruit's peel and pulp. Despite being a substantial fruit crop in tropical and subtropical areas of the world, wilt-resistant hybrid guava (Psidium guajava × Psidium molle; PGPM) has not yet been the subject of a thorough examination. This study's goal was to assess the expression of MYB in guava fruit pulp, roots, and seeds to predict its function by in silico analysis of the guava root transcriptome data.

Results: In the current study, we have mined the MYBs family of MYB genes from the transcriptome of the PGPM guava root. We have mined 15 distinct MYB transcription factor genes/transcripts viz MYB3, MYB4, MYB23, MYB86, MYB90, MYB308, MYB5, MYB82, MYB114, MYB6, MYB305, MYB44, MYB51, MYB46, and MYB330. From the analyses, it was found that R2-MYB and R3-MYB domains are conserved in all known guava MYB proteins. The expression of six different MYB TFs was examined using semi-quantitative RT-PCR in "Shweta" pulp (white colour pulp), "Lalit" pulp (red color pulp), "Lalit" root, and "Lalit" seed.

Conclusion: There were 15 MYB family members observed in guava. They were unequally distributed across the chromosomes, most likely as a result of gene duplication. Additionally, the expression patterns of the particular MYBs showed that MYB may be involved in the control of wilt, fruit ripening, seed development, and root development. Our results allow for a more thorough functional characterization of the guava MYB family genes and open the door to additional research into one essential MYB transcription factor family of genes and its involvement in the growth and ripening of guava fruit.

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抗枯萎番石榴MYB家族基因的硅挖掘与鉴定。
背景:MYB家族是植物中最重要的转录因子群之一。然而,有几种myb与次生代谢有关,对决定水果果皮和果肉的颜色很重要。尽管番石榴在世界热带和亚热带地区是一种重要的水果作物,但抗枯萎杂交番石榴(Psidium guajava × Psidium molle;PGPM)尚未成为彻底检查的主题。本研究的目的是评估MYB在番石榴果肉、根和种子中的表达,并通过对番石榴根转录组数据的计算机分析来预测其功能。结果:在目前的研究中,我们已经从PGPM番石榴根的转录组中挖掘出MYB基因家族。我们已经发现了15种不同的MYB转录因子基因/转录本,即MYB3、MYB4、MYB23、MYB86、MYB90、MYB308、MYB5、MYB82、MYB114、MYB6、MYB305、MYB44、MYB51、MYB46和MYB330。从分析中发现,在所有已知的番石榴MYB蛋白中,R2-MYB和R3-MYB结构域是保守的。采用半定量RT-PCR技术检测了6种不同MYB TFs在“Shweta”果肉(白色果肉)、“Lalit”果肉(红色果肉)、“Lalit”根和“Lalit”种子中的表达。结论:番石榴中有15个MYB家族成员。它们在染色体上的分布不均匀,很可能是基因复制的结果。此外,特定MYB的表达模式表明,MYB可能参与控制枯萎、果实成熟、种子发育和根系发育。我们的研究结果允许对番石榴MYB家族基因进行更彻底的功能表征,并为进一步研究一个重要的MYB转录因子家族基因及其在番石榴果实生长和成熟中的作用打开了大门。
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