Anti-fungal efficacy of Miswak Extract (Salvadora Persica) and commercial cleaner against Candida albicans on heat cured polymethylmethacrylate denture base.

IF 3.1 4区 医学 Q2 BIOPHYSICS Journal of Applied Biomaterials & Functional Materials Pub Date : 2023-01-01 DOI:10.1177/22808000231165666
Nadia Wajdan, Kashif Aslam, Rafat Amin, Sadaf Khan, Naseer Ahmed, Abhishek Lal, Eman M AlHamdan, Fahim Vohra, Tariq Abduljabbar, Artak Heboyan
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Abstract

Aim: The aim of the study was to compare the antifungal efficacy between commercial cleaner (Corega) and Miswak extract (Salvadora persica) against Candida albicans on heat cured Polymethyl methacrylate (PMMA) acrylic denture base resin.

Materials and methods: Forty-eight samples of heat cured PMMA acrylic denture base resin were fabircated in the study. The sterile acrylic resin specimens were immersed in standardized cell suspension of Candida albicans and incubated for 60 min at 370°C for cell adhesion and 2 h at 370°C for biofilm formation. After 24 h biofilm was evaluated by cell viability (CFUs) on SDA and cell counting of Candida albicans under light microscope at 400× magnification. The fungicidal effect of commercial cleaner and Miswak extract on Candida albicans biofilm was then evaluated by colony-forming units on SDA and cell counting under light microscope at 400× magnification.

Results: Screening test agar disk-diffusion assay showed mean inhibitory zone of 3 mm for commercial cleaner as compared to Miswak extract, which showed mean inhibitory zone of 2 and 1 mm for different concentrations. Broth microdilution method showed 31 mg/ml MIC and 62.5 mg/ml Minimal Fungicidal Concentration (MFC) values for commercial cleaner as compared to Miswak extract that showed 125 mg/ml MIC and 250 mg/ml MFC values against Candida albicans. A significant difference (p < 0.05) was observed between pre and post treatment of both commercial cleaner and Miswak extract, for CFUs and cell count for Candida albicans.

Conclusion: Commercial denture cleaner (Corega) showed better antifungal (C albicans) activity than Miswak extract (Salvadora persica) on heat cured PMMA acrylic denture base resin.

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Miswak 提取物(Salvadora Persica)和商用清洁剂对热固化聚甲基丙烯酸甲酯义齿基托上白色念珠菌的抗真菌功效。
目的:本研究旨在比较商用清洁剂(Corega)和 Miswak 提取物(Salvadora persica)对热固化聚甲基丙烯酸甲酯(PMMA)丙烯酸义齿基托树脂上白色念珠菌的抗真菌功效:本研究共制作了 48 份热固化 PMMA 丙烯酸义齿基托树脂样本。将无菌丙烯酸树脂试样浸入白色念珠菌的标准细胞悬浮液中,在 370°C 下培养 60 分钟以检测细胞粘附情况,在 370°C 下培养 2 小时以检测生物膜形成情况。24 小时后,通过 SDA 上的细胞存活率(CFUs)和 400 倍放大镜下的白色念珠菌细胞计数对生物膜进行评估。然后,通过 SDA 上的菌落形成单位和 400 倍放大光镜下的细胞计数,评估商用清洁剂和 Miswak 提取物对白念珠菌生物膜的杀菌效果:筛选试验琼脂盘扩散法显示,商用清洁剂的平均抑制区为 3 毫米,而不同浓度的 Miswak 提取物的平均抑制区分别为 2 毫米和 1 毫米。肉汤微量稀释法显示,商用清洁剂的 MIC 值为 31 毫克/毫升,最低杀菌浓度 (MFC) 值为 62.5 毫克/毫升,而 Miswak 提取物对白色念珠菌的 MIC 值为 125 毫克/毫升,最低杀菌浓度 (MFC) 值为 250 毫克/毫升。商用清洁剂和 Miswak 提取物在处理前和处理后对白色念珠菌的 CFUs 和细胞数都有明显差异(p < 0.05):在热固化 PMMA 丙烯酸义齿基底树脂上,商用义齿清洁剂(Corega)比 Miswak 提取物(Salvadora persica)显示出更好的抗真菌(白色念珠菌)活性。
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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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