The study of early screening technique for fruiting ability of Lentinula edodes hybrid progenies.

IF 1.5 4区 生物学 Q4 MYCOLOGY Mycoscience Pub Date : 2022-01-01 DOI:10.47371/mycosci.2022.06.001
Yu Li, Hailong Yu, Liangmin Li, Chunyan Song, Xiaodong Shang, Qi Tan, Lujun Zhang, Qiaozhen Li
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Abstract

Crossbreeding is the most commonly used method in breeding of Lentinula edodes, however low fruiting rate of the hybrids has always caused troubles and barriers for breeders. An early screening method of the fruiting ability could make the breeding work more efficient. In this paper, a rapid and high-throughput laccase activity detection method based on agar diffusion principle was developed. In this way, we investigated the constitutive and inducible extracellular laccase activity of 36 strains in a breeding population of L. edodes on different media and performed a correlation analysis with fruiting ability of these strains. The results showed the laccase activity of mycelium cultured in non-induced medium for 8 d could be used as an early screening index to judge whether it had fruiting ability at the later stage. Early rapid and simple screening method for hybrid populations was established based on laccase activity characteristics of mycelia. 127 strains from another 5 different hybrid populations were used to verify the early screening method. From the validation results, the early screening method was effective, but the appropriate screening threshold was needed to select according to the cross population, which would greatly to improve the breeding efficiency of L. edodes.

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香菇杂交后代结实能力的早期筛选技术研究。
杂交育种是香菇育种中最常用的方法,但杂种结实率低一直给育种工作者带来困扰和障碍。结果能力的早期筛选方法可以提高育种工作的效率。本文建立了一种基于琼脂扩散原理的快速、高通量漆酶活性检测方法。以此为基础,对36株L. edodes繁殖群体在不同培养基上的组成型和诱导型胞外漆酶活性进行了研究,并对其结实能力进行了相关分析。结果表明,在非诱导培养基中培养8 d的菌丝体漆酶活性可作为判断其后期是否具有结果力的早期筛选指标。建立了基于菌丝漆酶活性特征的杂交群体早期快速简便筛选方法。用另外5个不同杂交群体的127个菌株验证早期筛选方法。从验证结果来看,早期筛选方法是有效的,但需要根据杂交群体选择合适的筛选阈值,这将大大提高羊草的育种效率。
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来源期刊
Mycoscience
Mycoscience MYCOLOGY-
CiteScore
3.00
自引率
7.10%
发文量
32
审稿时长
3 months
期刊介绍: Mycoscience is the official English-language journal of the Mycological Society of Japan and is issued bimonthly. Mycoscience publishes original research articles and reviews on various topics related to fungi including yeasts and other organisms that have traditionally been studied by mycologists. The research areas covered by Mycoscience extend from such purely scientific fields as systematics, evolution, phylogeny, morphology, ecology, physiology, biochemistry, genetics, and molecular biology, to agricultural, medical, and industrial applications. New and improved applications of well-established mycological techniques and methods are also covered.
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