Screening bacterial isolates for biocontrol of sheath blight in rice plants.

IF 1.4 4区 农林科学 Q4 ENVIRONMENTAL SCIENCES Journal of Environmental Science and Health Part B-pesticides Food Contaminants and Agricultural Wastes Pub Date : 2023-01-01 Epub Date: 2023-06-14 DOI:10.1080/03601234.2023.2220644
Akintunde Abiodun Ajulo, Princewill Chukwuma Asobia, Rodrigo Silva de Oliveira, Gustavo de Andrade Bezerra, Ariany Rosa Gonçalves, Marta Cristina Corsi de Filippi
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Abstract

Sheath blight (Rhizoctonia solani) causes significant yield losses in rice (Oryza sativa L.). Its sustainable management needs an efficient biocontrol agent. The objective was to screen bacterial isolates as an antagonist to R. solani and identify the most efficient ones as sheath blight suppressors under greenhouse conditions. Two assays (E1 and E2) were performed in a completely randomized design with three replications. E1 tested 21 bacterial isolates antagonists to R. solani in vitro. E2 was conducted under greenhouse conditions, with rice cultivar BRS Pampeira sown in plastic pots (7 kg) containing fertilized soil. Sixty old plants were inoculated with a segment of a toothpick containing fragments of R. solani, followed by spray inoculation of a bacterial suspension (108 CFU/mL). The severity of the disease was determined by calculating the relative lesion size formed on the colm. Isolates BRM32112 (Pseudomonas nitroreducens), BRM65929 (Priestia megaterium), and BRM65919 (Bacillus cereus) reduced R. solani colony radial growth by 92.8, 77.56, and 75.56%, respectively while BRM63523 (Serratia marcescens), BRM65923 and BRM65916 (P. megaterium) and BRM65919 (B. cereus) with 23.45, 23.37, 23.62, and 20.17 cm, respectively were effective at suppressing sheath blight in greenhouse, indicating their potential as a biofungicide for sheath blight suppression.

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筛选用于生物防治水稻鞘枯病的细菌分离物。
鞘枯病(Rhizoctonia solani)给水稻(Oryza sativa L.)造成了严重的产量损失。其可持续管理需要高效的生物控制剂。本研究的目的是在温室条件下筛选拮抗根瘤鞘霉菌的细菌分离物,并确定最有效的鞘霉菌抑制剂。采用完全随机设计进行了两次试验(E1 和 E2),三次重复。E1 在体外测试了 21 种细菌分离物对 R. solani 的拮抗作用。E2 在温室条件下进行,水稻栽培品种 BRS Pampeira 播种在装有肥沃土壤的塑料盆(7 千克)中。60 株老植株接种了含有 R. solani 片段的牙签,然后喷洒了细菌悬浮液(108 CFU/mL)。通过计算茎柱上形成的相对病斑大小来确定病害的严重程度。分离菌 BRM32112(硝化假单胞菌)、BRM65929(巨苞假单胞菌)和 BRM65919(蜡样芽孢杆菌)分别减少了 92.8%、77.56% 和 75.56%的 R. solani 菌落径向生长量,而 BRM63523(肉豆蔻沙雷氏菌)、BRM65923 和 BRM65916(巨苞假单胞菌)以及 BRM65919(蜡样芽孢杆菌)则分别减少了 95.8%、77.56% 和 75.56%的 R. solani 菌落径向生长量。BRM63523(Serratia marcesens)、BRM65923 和 BRM65916(P.mgaterium)以及 BRM65919(B.cereus)对温室鞘枯病的抑制作用分别为 23.45 厘米、23.37 厘米、23.62 厘米和 20.17 厘米,表明它们具有作为生物杀菌剂抑制鞘枯病的潜力。
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来源期刊
CiteScore
4.00
自引率
5.00%
发文量
87
审稿时长
1 months
期刊介绍: 12 issues per year Abstracted/indexed in: Agricola; Analytical Abstracts; ASFA 3: Aquatic Pollution & Environmental Quality; BioSciences Information Service of Biological Abstracts (BIOSIS); CAB Abstracts; CAB AGBiotech News and Information; CAB Irrigation & Drainage Abstracts; CAB Soils & Fertilizers Abstracts; Chemical Abstracts Service Plus; CSA Aluminum Industry Abstracts; CSA ANTE: Abstracts in New Technology and Engineering; CSA ASFA 3 Aquatic Pollution and Environmental Quality; CSA ASSIA: Applied Social Sciences Index & Abstracts; CSA Ecology Abstracts; CSA Entomology Abstracts; CSA Environmental Engineering Abstracts; CSA Health & Safety Science Abstracts; CSA Pollution Abstracts; CSA Toxicology Abstracts; CSA Water Resource Abstracts; EBSCOhost Online Research Databases; Elsevier BIOBASE/Current Awareness in Biological Sciences; Elsevier Engineering Information: EMBASE/Excerpta Medica/ Engineering Index/COMPENDEX PLUS; Environment Abstracts; Environmental Knowledge; Food Science and Technology Abstracts; Geo Abstracts; Geobase; Food Science; Index Medicus/ MEDLINE; INIST-Pascal/ CNRS; NIOSHTIC; ISI BIOSIS Previews; Pesticides; Food Contaminants and Agricultural Wastes: Analytical Abstracts; Pollution Abstracts; PubSCIENCE; Reference Update; Research Alert; Science Citation Index Expanded (SCIE); and Water Resources Abstracts.
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