Involvement of RNA chaperone hfq in the regulation of antibiotic resistance and virulence in Shigella sonnei

IF 2.5 4区生物学 Q3 MICROBIOLOGY Research in microbiology Pub Date : 2023-06-01 DOI:10.1016/j.resmic.2023.104047

Ya Wang , Yanli Teng , Juan Geng , Jinzhao Long , Haiyan Yang , Guangcai Duan , Shuaiyin Chen

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引用次数: 2

Abstract

The host factor for RNA phage Qβ replicase (Hfq) is a crucial post-transcriptional regulator in many bacterial pathogens, facilitating the interaction between small non-coding RNAs (sRNAs) and their target mRNAs. Studies have suggested that Hfq plays a role in antibiotic resistance and virulence in bacteria, although its functions in Shigella are not fully understood. In this study, we investigated the functional roles of Hfq in Shigella sonnei (S. sonnei) by constructing an hfq deletion mutant. Our phenotypic assays showed that the hfq deletion mutant was more sensitivity to antibiotics and had impaired virulence. Transcriptome analyses supported the results concerning the phenotype of the hfq mutant and showed that differentially expressed genes were mainly enriched in the KEGG pathways two-component system, ABC transporters, ribosome, and Escherichia coli biofilm formation. Additionally, we predicted eleven novel Hfq-dependent sRNAs, which were potentially involved in the regulation of antibiotic resistance and/or virulence in S. sonnei. Our findings suggest that Hfq plays a post-transcriptional role in regulating antibiotic resistance and virulence in S. sonnei, and could provide a basis for future studies on Hfq-sRNA-mRNA regulatory networks in this important pathogen.

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RNA伴侣hfq参与索内志贺氏菌抗生素耐药性和毒力的调控

RNA噬菌体Qβ复制酶（Hfq）的宿主因子是许多细菌病原体中至关重要的转录后调节因子，促进小型非编码RNA（sRNA）与其靶mRNA之间的相互作用。研究表明，Hfq在细菌的抗生素耐药性和毒力中发挥作用，尽管它在志贺菌中的作用尚不完全清楚。在本研究中，我们通过构建一个Hfq缺失突变体来研究Hfq在宋氏志贺菌（S.sonnei）中的功能作用。我们的表型分析表明，hfq缺失突变体对抗生素更敏感，毒力受损。转录组分析支持了关于hfq突变体表型的结果，并表明差异表达基因主要富集在KEGG通路双组分系统、ABC转运蛋白、核糖体和大肠杆菌生物膜形成中。此外，我们预测了11种新的Hfq依赖性sRNA，它们可能参与调节宋尼链霉菌的抗生素耐药性和/或毒力，为今后研究Hfq-sRNAmRNA在该重要病原体中的调控网络提供了基础。

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来源期刊

Research in microbiology 生物-微生物学

CiteScore

4.10

自引率

3.80%

发文量

审稿时长

16 days

期刊介绍： Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.