Whole genome analyses for c-type cytochromes associated with respiratory chains in the extreme thermophile, Thermus thermophilus.

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2023-11-15 Epub Date: 2023-06-30 DOI:10.2323/jgam.2023.06.005
Koyu Hon-Nami, Atsushi Hijikata, Kei Yura, Yoshitaka Bessho
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引用次数: 0

Abstract

In thermophilic microorganisms, c-type cytochrome (cyt) proteins mainly function in the respiratory chain as electron carriers. Genome analyses at the beginning of this century revealed a variety of genes harboring the heme c motif. Here, we describe the results of surveying genes with the heme c motif, CxxCH, in a genome database comprising four strains of Thermus thermophilus, including strain HB8, and the confirmation of 19 c-type cytochromes among 27 selected genes. We analyzed the 19 genes, including the expression of four, by a bioinformatics approach to elucidate their individual attributes. One of the approaches included an analysis based on the secondary structure alignment pattern between the heme c motif and the 6th ligand. The predicted structures revealed many cyt c domains with fewer β-strands, such as mitochondrial cyt c, in addition to the β-strand unique to Thermus inserted in cyt c domains, as in T. thermophilus cyt c552 and caa3 cyt c oxidase subunit IIc. The surveyed thermophiles harbor potential proteins with a variety of cyt c folds. The gene analyses led to the development of an index for the classification of cyt c domains. Based on these results, we propose names for T. thermophilus genes harboring the cyt c fold.

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极端嗜热动物(Thermus thermoophilus)呼吸链相关c型细胞色素的全基因组分析。
在嗜热微生物中,c型细胞色素(cyt)蛋白主要作为电子载体在呼吸链中起作用。本世纪初的基因组分析揭示了多种含有血红素c基序的基因。在这里,我们描述了在包括菌株HB8在内的4株嗜热热菌基因组数据库中调查含有血红素c基序CxxCH基因的结果,并在27个选定的基因中确认了19个c型细胞色素。我们通过生物信息学方法分析了19个基因,其中包括4个基因的表达,以阐明它们的个体属性。其中一种方法包括基于血红素c基序与第6配体之间的二级结构比对模式的分析。预测的结构揭示了许多具有较少β-链的cyt c结构域,如线粒体cyt c,以及插入cyt c结构域的Thermus特有的β-链,如嗜热t细胞cyt c552和caa3 cyt c氧化酶亚基IIc。所调查的嗜热菌含有多种cyt - c折叠的潜在蛋白质。基因分析导致了cyt - c结构域分类指数的发展。基于这些结果,我们提出了嗜热T.的基因窝藏细胞c折叠的名称。
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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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