Application of Improved UNet and EnglightenGAN for Segmentation and Reconstruction of In Situ Roots.

IF 7.6 1区 农林科学 Q1 AGRONOMY Plant Phenomics Pub Date : 2023-01-01 DOI:10.34133/plantphenomics.0066
Qiushi Yu, Jingqi Wang, Hui Tang, Jiaxi Zhang, Wenjie Zhang, Liantao Liu, Nan Wang
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Abstract

The root is an important organ for crops to absorb water and nutrients. Complete and accurate acquisition of root phenotype information is important in root phenomics research. The in situ root research method can obtain root images without destroying the roots. In the image, some of the roots are vulnerable to soil shading, which severely fractures the root system and diminishes its structural integrity. The methods of ensuring the integrity of in situ root identification and establishing in situ root image phenotypic restoration remain to be explored. Therefore, based on the in situ root image of cotton, this study proposes a root segmentation and reconstruction strategy, improves the UNet model, and achieves precise segmentation. It also adjusts the weight parameters of EnlightenGAN to achieve complete reconstruction and employs transfer learning to implement enhanced segmentation using the results of the former two. The research results show that the improved UNet model has an accuracy of 99.2%, mIOU of 87.03%, and F1 of 92.63%. The root reconstructed by EnlightenGAN after direct segmentation has an effective reconstruction ratio of 92.46%. This study enables a transition from supervised to unsupervised training of root system reconstruction by designing a combination strategy of segmentation and reconstruction network. It achieves the integrity restoration of in situ root system pictures and offers a fresh approach to studying the phenotypic of in situ root systems, also realizes the restoration of the integrity of the in situ root image, and provides a new method for in situ root phenotype study.

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改进UNet和englighgan在原位树根分割与重建中的应用。
根是作物吸收水分和养分的重要器官。完整、准确地获取根表型信息是根表型组学研究的重要内容。原位根系研究方法可以在不破坏根系的情况下获得根系图像。在图像中,一些根系容易受到土壤阴影的影响,这严重破坏了根系,降低了其结构完整性。如何保证原位根系鉴定的完整性,建立原位根系图像表型恢复,仍有待探索。因此,本研究基于棉花原位根系图像,提出了一种根系分割重建策略,并对UNet模型进行了改进,实现了精确分割。调整了启蒙化gan的权重参数,实现了完全重构,并利用前两者的结果,利用迁移学习实现了增强分割。研究结果表明,改进后的UNet模型准确率为99.2%,mIOU为87.03%,F1为92.63%。启蒙gan直接分割后重建的根的有效重建率为92.46%。本研究通过设计一种分割与重建网络相结合的策略,实现了根系重建训练从监督到无监督的过渡。实现了原位根系图像的完整还原,为原位根系表型研究提供了一种新的途径,也实现了原位根系图像的完整还原,为原位根系表型研究提供了一种新的方法。
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来源期刊
Plant Phenomics
Plant Phenomics Multiple-
CiteScore
8.60
自引率
9.20%
发文量
26
审稿时长
14 weeks
期刊介绍: Plant Phenomics is an Open Access journal published in affiliation with the State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University (NAU) and published by the American Association for the Advancement of Science (AAAS). Like all partners participating in the Science Partner Journal program, Plant Phenomics is editorially independent from the Science family of journals. The mission of Plant Phenomics is to publish novel research that will advance all aspects of plant phenotyping from the cell to the plant population levels using innovative combinations of sensor systems and data analytics. Plant Phenomics aims also to connect phenomics to other science domains, such as genomics, genetics, physiology, molecular biology, bioinformatics, statistics, mathematics, and computer sciences. Plant Phenomics should thus contribute to advance plant sciences and agriculture/forestry/horticulture by addressing key scientific challenges in the area of plant phenomics. The scope of the journal covers the latest technologies in plant phenotyping for data acquisition, data management, data interpretation, modeling, and their practical applications for crop cultivation, plant breeding, forestry, horticulture, ecology, and other plant-related domains.
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