An idea to explore: Engaging high school students in structure-function studies of bacterial sortase enzymes and inhibitors - A comprehensive computational experimental pipeline

IF 1.2 4区 教育学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry and Molecular Biology Education Pub Date : 2023-07-18 DOI:10.1002/bmb.21769
Shivani Godse, Tanvi Sapar, Jeanine F. Amacher
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引用次数: 0

Abstract

High school science fairs provide an exceptional opportunity for students to gain experience with scientific research, and participation has positive outcomes with respect to chosen careers in the sciences. However, it can be challenging to engage high school students in university-level research outside of formal internship programs. Here, we describe an experimental pipeline for a computational structural biology project that engages high school students. Students are involved at every step of the investigation and utilize freely available software to dock inhibitors onto protein homologues, and then analyze the resulting complexes. Bacterial sortases are transpeptidases on the cell surface of Gram-positive bacteria and are a potential target for the development of antibiotics. Students modeled inhibitors bound to sortases from several organisms, asking questions about affinity and selectivity. Their project was ranked in the top 10% at both regional and state science fairs. This project design is easily adaptable to countless other protein systems and provides a pipeline for collaborative high school student/university professor inquiry.

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一个探索的想法:让高中生参与细菌分选酶和抑制剂的结构-功能研究-一个全面的计算实验管道。
高中科学博览会为学生提供了一个获得科学研究经验的绝佳机会,参与其中对选择科学领域的职业有积极的影响。然而,在正式的实习项目之外,让高中生参与大学水平的研究可能是一项挑战。在这里,我们描述了一个实验管道的计算结构生物学项目,从事高中学生。学生们参与了研究的每一步,并利用免费的软件将抑制剂与蛋白质同源物对接,然后分析得到的复合物。细菌分选酶是革兰氏阳性细菌细胞表面的转肽酶,是开发抗生素的潜在靶点。学生们模拟了与几种生物的分选酶结合的抑制剂,并提出了关于亲和力和选择性的问题。他们的项目在地区和国家科学展览中都名列前10%。该项目设计很容易适用于无数其他蛋白质系统,并为高中学生/大学教授的协作查询提供了一个管道。
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来源期刊
Biochemistry and Molecular Biology Education
Biochemistry and Molecular Biology Education 生物-生化与分子生物学
CiteScore
2.60
自引率
14.30%
发文量
99
审稿时长
6-12 weeks
期刊介绍: The aim of BAMBED is to enhance teacher preparation and student learning in Biochemistry, Molecular Biology, and related sciences such as Biophysics and Cell Biology, by promoting the world-wide dissemination of educational materials. BAMBED seeks and communicates articles on many topics, including: Innovative techniques in teaching and learning. New pedagogical approaches. Research in biochemistry and molecular biology education. Reviews on emerging areas of Biochemistry and Molecular Biology to provide background for the preparation of lectures, seminars, student presentations, dissertations, etc. Historical Reviews describing "Paths to Discovery". Novel and proven laboratory experiments that have both skill-building and discovery-based characteristics. Reviews of relevant textbooks, software, and websites. Descriptions of software for educational use. Descriptions of multimedia materials such as tutorials on various aspects of biochemistry and molecular biology.
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