Fresh human amniotic membrane wrapping promotes peripheral nerve regeneration in PGA-collagen tubes.

IF 1 4区医学 Q3 ORTHOPEDICS Journal of Plastic Surgery and Hand Surgery Pub Date : 2023-05-23 DOI:10.2340/jphs.v58.6496

Atsuhiko Iwao, Hiroto Saijo, Takafumi Nakayama, Akihito Higashi, Kazuya Kashiyama, Norisato Mitsutake, Katsumi Tanaka

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引用次数: 1

Abstract

Background: An artificial nerve conduit can interpose the peripheral nerve defect without donor site morbidity. However, treatment outcomes are often unsatisfactory. Human amniotic membrane (HAM) wrapping has been reported to promote peripheral nerve regeneration. We evaluated the effects of a combined application of fresh HAM wrapping and a polyglycolic acid tube filled with collagen (PGA-c) in a rat sciatic nerve 8-mm defect model.

Methods: The rats were divided into three groups: (1) the PGA-c group (n = 5), in which the gap was interposed with the PGA-c; (2) the PGA-c/HAM group (n = 5), in which the gap was interposed with the PGA-c bridge, then HAM (14 × 7 mm) was wrapped around it; and (3) the Sham group (n = 5). Walking-Track recovery, electromyographic recovery, and histological recovery of the regenerated nerve were evaluated at 12 weeks postoperatively.

Results: Compared to the PGA-c group, the PGA-c/HAM group showed significantly better recovery in terminal latency (3.4 ± 0.31 ms vs. 6.6 ± 0.72 ms, p < 0.001), compound muscle action potential (0.19 ± 0.025 mV vs. 0.072 ± 0.027 mV, p < 0.01), myelinated axon perimeter (15 ± 1.3 μm vs. 8.7 ± 0.63 μm, p < 0.01), and g-ratio (0.69 ± 0.0089 vs. 0.78 ± 0.014, p < 0.001).

Conclusion: This combined application highly promotes peripheral nerve regeneration and may be more useful than PGA-c alone.

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新鲜人羊膜包裹促进pga -胶原管内周围神经再生。

背景:人工神经导管可以介入周围神经缺损而不引起供区病变。然而，治疗结果往往不令人满意。人羊膜(HAM)包裹有促进周围神经再生的报道。我们在大鼠8毫米坐骨神经缺损模型中评估了新鲜火腿包膜和填充胶原蛋白的聚乙醇酸管(PGA-c)联合应用的效果。方法:将大鼠分为三组:(1)PGA-c组(n = 5)，用PGA-c填充间隙;(2) PGA-c/HAM组(n = 5)，在间隙中插入PGA-c桥，然后在间隙周围包裹HAM (14 × 7 mm);(3)假手术组(n = 5)。术后12周观察再生神经的步行轨迹恢复、肌电图恢复和组织学恢复情况。结果:与PGA-c组相比，PGA-c/HAM组在终末潜伏期(3.4±0.31 ms比6.6±0.72 ms, p < 0.001)、复合肌动作电位(0.19±0.025 mV比0.072±0.027 mV, p < 0.01)、髓鞘轴突周长(15±1.3 μm比8.7±0.63 μm, p < 0.01)和g比(0.69±0.0089比0.78±0.014,p < 0.001)方面的恢复明显优于PGA-c组。结论:与PGA-c单独应用相比，PGA-c联合应用可显著促进周围神经再生。

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来源期刊

Journal of Plastic Surgery and Hand Surgery ORTHOPEDICS-SURGERY

CiteScore

2.10

自引率

0.00%

发文量

108

审稿时长

6-12 weeks

期刊介绍： The purpose of the Journal of Plastic Surgery and Hand Surgery is to serve as an international forum for plastic surgery, hand surgery and related research. Interest is focused on original articles on basic research and clinical evaluation. The scope of the journal comprises: • Articles concerning operative methods and follow-up studies • Research articles on subjects related to plastic and hand surgery • Articles on cranio-maxillofacial surgery, including cleft lip and palate surgery. Extended issues are published occasionally, dealing with special topics such as microvascular surgery, craniofacial surgery, or burns. Supplements, usually doctoral theses, may also be published. The journal is published for the Acta Chirurgica Scandinavica society and sponsored by the Key Foundation, Sweden. The journal was previously published as Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery.