Solvent preextraction influenced to coumarin and glucose binding capacity of cinnamomi's extracts.

IF 1.4 Q3 Pharmacology, Toxicology and Pharmaceutics Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2023-04-01 Epub Date: 2023-04-13 DOI:10.4103/japtr.japtr_671_22
Martha Ervina, Virgiana Kristin Coco Wea, Annisah Oktaviani, Lanny Hartanti, Restry Sinansari, Yufita Ratnasari Wilianto, Steven, Caroline
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Abstract

Cinnamon is one of the world's oldest spices that is also widely used as medicine for antimicrobe, anti-inflammation, and immune stimulant to now developed as an antidiabetic herbal medicine. Among its bioactive contents, the coumarin must have been controlled; since of its toxicities. Therefore, in this study aimed to reduce the amount of coumarin in the extracts by chloroform preextraction to dried powdered cinnamons and determine their glucose binding capacity. The extractions were used two methods by water infusion and ethanol soxhletation. To measure the coumarin's was used validated thin-layer chromatography (TLC)-densitometry, while for the chemical profiling of the extract was examined with liquid chromatography-mass spectrometry. The activity antidiabetic of the extracts was obtained by glucose binding. The TLC-densitometry method has been validated with silica gel 60F254 and n-hexane: ethyl acetate (8:2, v/v) systems. The coumarin's spot was observed at a wavelength of 285 nm on retention factor (Rf) 0.33, with tailings factor 1. The intraday and interday linearities tests showed a linear response result. The recovery value, coefficient of variation, and detection and a quantitation limit were met the standard requirements, respectively. Moreover, the results were observed (1) the solvent preextraction may reduce the coumarin content, (2) the coumarin content in the ethanol extract was higher than in the infusion, and (3) the preextraction solvents would reduce the glucose-binding capacity in ethanol and water cinnamomi's extract. These results may be developed further and applied for producing cinnamon's free coumarin extracts.

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溶剂预处理对肉桂提取物香豆素和葡萄糖结合能力的影响。
肉桂是世界上最古老的香料之一,也被广泛用作抗微生物、抗炎和免疫兴奋剂的药物,现在被开发为抗糖尿病的草药。在其生物活性成分中,香豆素必须受到控制;由于其毒性。因此,本研究旨在通过氯仿预牵引干燥的肉桂粉来减少提取物中香豆素的含量,并测定其葡萄糖结合能力。提取液采用水浸泡法和乙醇浸泡法两种方法。香豆素的测定采用经验证的薄层色谱(TLC)-密度计,而提取物的化学分析采用液相色谱-质谱法。通过葡萄糖结合获得提取物的抗糖尿病活性。用硅胶60F254和正己烷∶乙酸乙酯(8:2,v/v)体系对TLC密度测定方法进行了验证。在保留因子(Rf)0.33、尾渣因子为1的285nm波长下观察到香豆素斑点。日内和日间线性测试显示出线性响应结果。回收值、变异系数、检测限和定量限分别符合标准要求。此外,观察到(1)溶剂预牵引可能降低香豆素含量,(2)乙醇提取物中的香豆素含量高于输液中的香豆素,以及(3)预牵引溶剂会降低乙醇和水肉桂提取物中的葡萄糖结合能力。这些结果可以进一步发展并应用于生产肉桂的游离香豆素提取物。
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来源期刊
CiteScore
2.00
自引率
7.10%
发文量
44
审稿时长
20 weeks
期刊介绍: Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is an Official Publication of Society of Pharmaceutical Education & Research™. It is an international journal published Quarterly. Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is available in online and print version. It is a peer reviewed journal aiming to communicate high quality original research work, reviews, short communications, case report, Ethics Forum, Education Forum and Letter to editor that contribute significantly to further the scientific knowledge related to the field of Pharmacy i.e. Pharmaceutics, Pharmacology, Pharmacognosy, Pharmaceutical Chemistry. Articles with timely interest and newer research concepts will be given more preference.
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