Serum, interstitial and sweat ATP in humans exposed to heat stress: Insights into roles of ATP in the heat loss responses

IF 1.3 4区 医学 Q4 PHYSIOLOGY Clinical Physiology and Functional Imaging Pub Date : 2023-05-04 DOI:10.1111/cpf.12825
Naoto Fujii, Yoko Tanabe, Tatsuro Amano, Koichi Watanabe, Narihiko Kondo, Takeshi Nishiyasu, Glen P. Kenny
{"title":"Serum, interstitial and sweat ATP in humans exposed to heat stress: Insights into roles of ATP in the heat loss responses","authors":"Naoto Fujii,&nbsp;Yoko Tanabe,&nbsp;Tatsuro Amano,&nbsp;Koichi Watanabe,&nbsp;Narihiko Kondo,&nbsp;Takeshi Nishiyasu,&nbsp;Glen P. Kenny","doi":"10.1111/cpf.12825","DOIUrl":null,"url":null,"abstract":"<p>Hyperthermia increases intravascular adenosine triphosphate (ATP) and is associated with greater hyperthermia-induced cutaneous vasodilation. Hyperthermia may also increase skin interstitial fluid ATP thereby activating cutaneous vascular smooth muscle cells and sweat glands. We evaluated the hypothesis that whole-body heating would increase skin interstitial fluid ATP, and this response would be associated with an increase in cutaneous vasodilation and sweating. Nineteen (8 females) young adults underwent whole-body heating using a water-perfusion suit to increase core temperature by ~1°C during which time cutaneous vascular conductance (CVC, ratio of laser-Doppler blood flow to mean arterial pressure) and sweat rate (ventilated capsule technique) were measured at four forearm skin sites to minimize between-site variations. Dialysate from the skin sites were collected via intradermal microdialysis. Heating increased serum ATP, CVC, and sweat rate (all <i>p</i> ≤ 0.031). However, heating did not modulate dialysate ATP (median, baseline vs. end-heating: 2.38 vs. 2.70 nmol/ml) (<i>p</i> = 0.068), though the effect size was moderate (Cohen's d = 0.566). While the heating-induced increase in CVC was not correlated with changes in serum ATP (<i>r</i> = 0.439, <i>p</i> = 0.060), we observed a negative correlation (<i>r</i><sub>s</sub> = −0.555, <i>p</i> = 0.017) between dialysate ATP and CVC. We did not observe a significant correlation between the heating-induced sweating and serum, dialysate, or sweat ATP (<i>r</i><sub>s</sub> = 0.091 to −0.322, all <i>p</i> ≥ 0.222). Altogether, we showed that passive heating increases ATP in blood and possibly skin interstitial fluid, with the latter potentially blunting cutaneous vasodilation. However, ATP does not appear to modulate sweating.</p>","PeriodicalId":10504,"journal":{"name":"Clinical Physiology and Functional Imaging","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2023-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical Physiology and Functional Imaging","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/cpf.12825","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHYSIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Hyperthermia increases intravascular adenosine triphosphate (ATP) and is associated with greater hyperthermia-induced cutaneous vasodilation. Hyperthermia may also increase skin interstitial fluid ATP thereby activating cutaneous vascular smooth muscle cells and sweat glands. We evaluated the hypothesis that whole-body heating would increase skin interstitial fluid ATP, and this response would be associated with an increase in cutaneous vasodilation and sweating. Nineteen (8 females) young adults underwent whole-body heating using a water-perfusion suit to increase core temperature by ~1°C during which time cutaneous vascular conductance (CVC, ratio of laser-Doppler blood flow to mean arterial pressure) and sweat rate (ventilated capsule technique) were measured at four forearm skin sites to minimize between-site variations. Dialysate from the skin sites were collected via intradermal microdialysis. Heating increased serum ATP, CVC, and sweat rate (all p ≤ 0.031). However, heating did not modulate dialysate ATP (median, baseline vs. end-heating: 2.38 vs. 2.70 nmol/ml) (p = 0.068), though the effect size was moderate (Cohen's d = 0.566). While the heating-induced increase in CVC was not correlated with changes in serum ATP (r = 0.439, p = 0.060), we observed a negative correlation (rs = −0.555, p = 0.017) between dialysate ATP and CVC. We did not observe a significant correlation between the heating-induced sweating and serum, dialysate, or sweat ATP (rs = 0.091 to −0.322, all p ≥ 0.222). Altogether, we showed that passive heating increases ATP in blood and possibly skin interstitial fluid, with the latter potentially blunting cutaneous vasodilation. However, ATP does not appear to modulate sweating.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
暴露于热应激的人的血清、间质和汗液ATP: ATP在热损失反应中的作用
高温可增加血管内三磷酸腺苷(ATP),并与高温诱导的皮肤血管舒张有关。热疗还可以增加皮肤间质液ATP,从而激活皮肤血管平滑肌细胞和汗腺。我们评估了全身加热会增加皮肤间质液ATP的假设,这种反应与皮肤血管舒张和出汗的增加有关。19名(8名女性)年轻人使用水灌注服进行全身加热,将核心温度提高约1°C,在此期间,测量前臂四个皮肤部位的皮肤血管电导(CVC,激光多普勒血流与平均动脉压的比率)和出汗率(通气胶囊技术),以最大限度地减少部位间的变化。通过皮内微透析收集皮肤部位的透析液。加热可增加血清ATP、CVC和出汗率(均p ≤ 0.031)。然而,加热并没有调节透析液ATP(中位数、基线与末端加热:2.38 vs.2.70 nmol/ml)(p = 0.068),尽管影响大小适中(Cohen的d = 0.566)。而加热引起的CVC升高与血清ATP的变化无关(r = 0.439,p = 0.060),我们观察到负相关(rs = −0.555,p = 0.017)。我们没有观察到加热引起的出汗与血清、透析液或汗液ATP(rs)之间的显著相关性 = 0.091至−0.322,所有p ≥ 0.222)。总之,我们发现被动加热会增加血液中的ATP,可能还会增加皮肤间质液,后者可能会减弱皮肤血管舒张。然而,ATP似乎并不能调节出汗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
3.40
自引率
5.60%
发文量
62
审稿时长
6-12 weeks
期刊介绍: Clinical Physiology and Functional Imaging publishes reports on clinical and experimental research pertinent to human physiology in health and disease. The scope of the Journal is very broad, covering all aspects of the regulatory system in the cardiovascular, renal and pulmonary systems with special emphasis on methodological aspects. The focus for the journal is, however, work that has potential clinical relevance. The Journal also features review articles on recent front-line research within these fields of interest. Covered by the major abstracting services including Current Contents and Science Citation Index, Clinical Physiology and Functional Imaging plays an important role in providing effective and productive communication among clinical physiologists world-wide.
期刊最新文献
Relationship between heart rate variability and echocardiography indices of cardiac function in healthy individuals. Plantar flexor strength and size decrease following single-leg disuse in uninjured adults: A meta-analysis. The effect of eccentric arm cycling on muscle damage and injury-related biomarkers. Issue Information COVID-19 is associated with cardiac structural and functional remodelling in healthy middle-aged and older individuals.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1