Modification of the second PEP4-allele facilitates an industrial Saccharomyces cerevisiae to tolerate tartaric acid stress

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2023-11-01 DOI:10.1016/j.resmic.2023.104109
Hongbo Zhang, Xiaomei Yang, Chi Shen, Jianqiu Sun, Yuhang Lu, Wanting Hu, Hongfei Yao, Wenhao Zhao
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Abstract

The practical significance of constructing robust industrial production strains against organic acid stress lies not only in improving fermentation efficiency but also in reducing manufacturing costs. In a previous study, we constructed an industrial Saccharomyces cerevisiae strain by modifying another PEP4-allele of a mutant that already had one PEP4-allele disrupted. This modification enhanced cellular tolerance to citric acid stress during growth. Unlike citric acid, which S. cerevisiae can consume, tartaric acid is often added to grape must during winemaking to increase total acidity and is not metabolizable. The results of the present study indicate that the modification of the second PEP4-allele improves the cellular tolerance of the strain with one PEP4-allele disrupted against tartaric acid stress during growth and contributes to maintaining intracellular pH homeostasis in cells subjected to tartaric acid stress. Moreover, under tartaric acid stress, a significant improvement in glucose-ethanol conversion performance, conferred by the modification of the second PEP4-allele, was observed. This study not only broadens our understanding of the role of the PEP4-allele in cellular regulation but also provides a prospective approach to reducing the concentration of sulfur dioxide used in winemaking.

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第二个PEP4等位基因的修饰促进工业酿酒酵母耐受酒石酸应激。
构建抗有机酸胁迫的强大工业生产菌株的实际意义不仅在于提高发酵效率,还在于降低生产成本。在之前的一项研究中,我们通过修饰一个突变株的另一个PEP4等位基因构建了一个工业酿酒酵母菌株。这种修饰增强了细胞在生长过程中对柠檬酸胁迫的耐受性。与酿酒酵母可以消耗的柠檬酸不同,酒石酸通常在酿酒过程中添加到葡萄汁中,以增加总酸度,并且不可代谢。本研究的结果表明,第二个PEP4等位基因的修饰提高了菌株的细胞耐受性,其中一个PEP4等位基因在生长过程中对酒石酸胁迫被破坏,并有助于在受到酒石酸胁迫的细胞中维持细胞内pH稳态。此外,在酒石酸胁迫下,观察到由第二个PEP4等位基因的修饰所赋予的葡萄糖-乙醇转化性能的显著改善。这项研究不仅拓宽了我们对PEP4等位基因在细胞调节中的作用的理解,而且为降低酿酒中使用的二氧化硫浓度提供了一种前瞻性的方法。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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