Osteogenic effect and mechanism of IL-10 in diabetic rat jaw defect mode.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Oral diseases Pub Date : 2024-05-01 Epub Date: 2023-08-08 DOI:10.1111/odi.14707

Sitong Hu, Yihui Zhu, Shujia Yu, Yanchuan Guo, Yihu Wang, Menghao Lv, Wei Bai, Pan Ma

{"title":"Osteogenic effect and mechanism of IL-10 in diabetic rat jaw defect mode.","authors":"Sitong Hu, Yihui Zhu, Shujia Yu, Yanchuan Guo, Yihu Wang, Menghao Lv, Wei Bai, Pan Ma","doi":"10.1111/odi.14707","DOIUrl":null,"url":null,"abstract":"Objective: The aim of this study was to investigate the effect of IL-10 on the phenotype polarization of macrophages and osteogenesis in diabetes mellitus type 2 (T2DM) rat jaw defects.Methods: Lipopolysaccharide (LPS) and interleukin-10 (IL-10) were chosen to induce the polarization of macrophages. In vitro assessment included wound-healing assay, western blotting, and alizarin red staining after co-culture of the bone marrow-derived mesenchymal stem cells (BMSCs) and induced macrophages. For in vivo study, IL-10 was loaded on GelMA-Heparin and applied to bone defects of the alveolar ridge in diabetic rats, while Bio-Oss Collagen, simple GelMA-Heparin, and blank control groups were set for contrast experiment. The mandibles of rats were processed for micro-computed tomography, histology, and immunohistochemistry 1 week and 4 weeks after the operation.Results: IL-10 induced expression of arginase 1, TGF-β1, EGR2, and Mannose Receptor (CD206), whereas LPS induced expression of iNOS, TNF-α, IL-6, CD80. The BMSCs co-cultured with macrophages induced by IL-10 showed increased migration, osteogenic differentiation, and mineralization in vitro. Notably, the IL-10-laden GelMA-Heparin group showed quicker new bone formation and a higher M2/M1 ratio of macrophages in the jawbone defect area compared with the control groups.Conclusions: IL-10 can stably induce macrophages to M2 type, thereby influencing BMSCs and improving the osteogenesis of jaw bone defects.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oral diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/odi.14707","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/8/8 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: The aim of this study was to investigate the effect of IL-10 on the phenotype polarization of macrophages and osteogenesis in diabetes mellitus type 2 (T2DM) rat jaw defects.

Methods: Lipopolysaccharide (LPS) and interleukin-10 (IL-10) were chosen to induce the polarization of macrophages. In vitro assessment included wound-healing assay, western blotting, and alizarin red staining after co-culture of the bone marrow-derived mesenchymal stem cells (BMSCs) and induced macrophages. For in vivo study, IL-10 was loaded on GelMA-Heparin and applied to bone defects of the alveolar ridge in diabetic rats, while Bio-Oss Collagen, simple GelMA-Heparin, and blank control groups were set for contrast experiment. The mandibles of rats were processed for micro-computed tomography, histology, and immunohistochemistry 1 week and 4 weeks after the operation.

Results: IL-10 induced expression of arginase 1, TGF-β1, EGR2, and Mannose Receptor (CD206), whereas LPS induced expression of iNOS, TNF-α, IL-6, CD80. The BMSCs co-cultured with macrophages induced by IL-10 showed increased migration, osteogenic differentiation, and mineralization in vitro. Notably, the IL-10-laden GelMA-Heparin group showed quicker new bone formation and a higher M2/M1 ratio of macrophages in the jawbone defect area compared with the control groups.

Conclusions: IL-10 can stably induce macrophages to M2 type, thereby influencing BMSCs and improving the osteogenesis of jaw bone defects.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

IL-10在糖尿病大鼠颌骨缺损模式中的成骨效应和机制

研究目的本研究旨在探讨IL-10对糖尿病2型（T2DM）大鼠颌骨缺损中巨噬细胞表型极化和成骨的影响：方法：选择脂多糖（LPS）和白细胞介素-10（IL-10）来诱导巨噬细胞的极化。体外评估包括伤口愈合试验、Western 印迹和骨髓间充质干细胞（BMSCs）与诱导巨噬细胞共培养后的茜素红染色。在体内研究中，IL-10 被负载在 GelMA-Heparin 上，应用于糖尿病大鼠牙槽脊骨缺损处，而 Bio-Oss 胶原组、单纯 GelMA-Heparin 组和空白对照组则用于对比实验。术后 1 周和 4 周，对大鼠下颌骨进行显微计算机断层扫描、组织学和免疫组化处理：结果：IL-10 诱导精氨酸酶 1、TGF-β1、EGR2 和甘露糖受体（CD206）的表达，而 LPS 诱导 iNOS、TNF-α、IL-6 和 CD80 的表达。经 IL-10 诱导的 BMSCs 与巨噬细胞共培养后，体外迁移、成骨分化和矿化均有所增加。值得注意的是，与对照组相比，添加了 IL-10 的 GelMA-Heparin 组新骨形成更快，颌骨缺损区巨噬细胞的 M2/M1 比率更高：结论：IL-10 可将巨噬细胞稳定诱导为 M2 型，从而影响 BMSCs 并改善颌骨缺损的成骨过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.