Pollen viabilities and gene expression profiles across Musa genomes.

IF 2.6 3区生物学 Q2 ECOLOGY AoB Plants Pub Date : 2023-07-01 DOI:10.1093/aobpla/plad052

Yonlada Mingmanit, Thanita Boonsrangsom, Kawee Sujipuli, Kumrop Ratanasut, Phithak Inthima

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Abstract

Banana (Musa spp.) is a major global economic fruit crop. However, cross-pollination from other Musa cultivars grown in nearby plantations results in seeded fruit that exceeds market demand. This study investigated pollen viability and germination and examined the expression profiles of pollen development-related genes across seven Musa genomes (AA, BB, AAA, BBB, AAB, ABB and ABBB). Twenty-three Musa cultivars were assessed for pollen viability using lacto-aceto-orcein and triphenyltetrazolium chloride staining methods. Results revealed that pollen viability obtained from both methods was significantly different among all the studied cultivars. Cultivars carrying BB (diploid) genomes had higher viability percentages than AA (diploid), AAA, BBB, AAB and ABB (triploid) and ABBB (tetraploid) genomes. Germination of the studied cultivars was also investigated on pollen culture medium, with results showing significant differences between the pollen of each cultivar. The best germinating cultivar was TKM (11.0 %), carrying BB genome. Expression profiles of pollen development-related genes by RT-qPCR indicated that both TPD1A and MYB80 genes were highly expressed in triploid Musa genomes but the PTC1 gene showed down-regulated expression, resulting in non-viable pollen. Pollen viability, pollen germination and pollen development-related genes differed across Musa cultivars. This knowledge will be useful for the selection of male parents for Musa cross-breeding programs. Pollen viability should also be considered when planning Musa production to avoid seeded fruit.

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油菜基因组花粉活力和基因表达谱。

香蕉(Musa spp.)是全球主要的经济水果作物。然而，与附近种植园种植的其他穆萨品种的异花授粉产生的有籽果实超过了市场需求。本研究研究了7个Musa基因组(AA、BB、AAA、BBB、AAB、ABB和ABBB)花粉活力和萌发情况，并检测了花粉发育相关基因的表达谱。采用乳酸-乙酰胆碱染色法和三苯四唑氯化铵染色法对23个Musa品种的花粉活力进行了评价。结果表明，两种方法测得的花粉活力在不同品种间差异显著。携带BB(二倍体)基因组的品种存活率高于AA(二倍体)、AAA、BBB、AAB、ABB(三倍体)和ABBB(四倍体)基因组。在花粉培养基上对不同品种的花粉萌发情况进行了研究，结果表明不同品种的花粉存在显著差异。发芽率最高的品种是携带BB基因组的TKM(11.0%)。RT-qPCR分析花粉发育相关基因的表达谱表明，TPD1A和MYB80基因在三倍体穆萨基因组中均有高表达，而PTC1基因表达下调，导致花粉不活。花粉活力、花粉萌发和花粉发育相关基因在不同品种间存在差异。这些知识将有助于穆萨杂交育种中雄性亲本的选择。在规划穆萨生产时也应考虑花粉活力，以避免种子果实。

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来源期刊

AoB Plants PLANT SCIENCES-

CiteScore

4.80

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： AoB PLANTS is an open-access, online journal that has been publishing peer-reviewed articles since 2010, with an emphasis on all aspects of environmental and evolutionary plant biology. Published by Oxford University Press, this journal is dedicated to rapid publication of research articles, reviews, commentaries and short communications. The taxonomic scope of the journal spans the full gamut of vascular and non-vascular plants, as well as other taxa that impact these organisms. AoB PLANTS provides a fast-track pathway for publishing high-quality research in an open-access environment, where papers are available online to anyone, anywhere free of charge.