Expression of MicroRNA-200a/b/c in the Mediobasal Hypothalamic Nuclei with Aging.

MicroRNA (Shariqah, United Arab Emirates) Pub Date : 2023-01-01 DOI:10.2174/2211536612666230810094531

Valentina V Porseva, Lydia G Pankrasheva, Konstantin Yu Moiseev, Polina A Anfimova, Andrey I Emanuilov, Nikolay Yu Levshin, Andrey A Baranov, Petr M Masliukov

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Abstract

Background: MicroRNAs (miRNAs) belong to small non-coding RNAs that coordinate the expression of cellular genes at the post-transcriptional level. The hypothalamus is a key regulator of homeostasis, biological rhythms and adaptation to different environmental factors. It also participates in the aging regulation. Variations in miRNA expression in the hypothalamus can affect the aging process.

Objective: Our objective of this study is to examine the expression of miR-200a-3p, miR-200b-3p, miR-200c-3p in the dorsomedial (DMN), ventromedial (VMN) and arcuate (ARN) nuclei of the hypothalamus in male and female rats during aging.

Methods: The expression of miR-200a-3p, miR-200b-3p, and miR-200c-3p in DMN, VMN and ARN was studied by qPCR-RT. The results were presented using the 2^-ΔΔCq algorithm.

Results: The expression of miR-200a-3p, miR-200b-3p, miR-200c-3p microRNAs decreases with aging in the DMN of males and in the VMN of females. The level of miR-200b-3p expression decreased in aged males in the VMN and females in the DMN. The expression of miR-200c-3p declined in aged males in the ARN and in females in the DMN. The expression of miR-200a-3p, miR-200b-3p, and miR-200c-3p did not change in females in the ARN in aging.

Conclusion: We found a decrease in the expression of members of the miR-200a-3p, miR-200b-3p, and miR-200c-3p in the tuberal hypothalamic nuclei and their sex differences in aging rats.

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衰老过程中下丘脑中基底核中MicroRNA-200a/b/c的表达

背景:MicroRNAs (miRNAs)属于小的非编码rna，在转录后水平协调细胞基因的表达。下丘脑是体内平衡、生物节律和适应不同环境因素的关键调节器。它还参与了衰老调节。下丘脑miRNA表达的变化可以影响衰老过程。目的:我们的研究目的是检测miR-200a-3p、miR-200b-3p、miR-200c-3p在雄性和雌性大鼠下丘脑背内侧核(DMN)、腹内侧核(VMN)和弓状核(ARN)衰老过程中的表达。方法:采用qPCR-RT检测miR-200a-3p、miR-200b-3p、miR-200c-3p在DMN、VMN和ARN中的表达情况。使用2-ΔΔCq算法给出了结果。结果:miR-200a-3p、miR-200b-3p、miR-200c-3p微rna在男性DMN和女性VMN中的表达随年龄增长而降低。miR-200b-3p在老年男性VMN和女性DMN中表达水平下降。miR-200c-3p在老年男性的ARN和女性的DMN中表达下降。随着年龄的增长，女性ARN中miR-200a-3p、miR-200b-3p和miR-200c-3p的表达没有变化。结论:我们发现衰老大鼠下丘脑结节核中miR-200a-3p、miR-200b-3p和miR-200c-3p成员表达减少，性别差异明显。

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MicroRNA (Shariqah, United Arab Emirates) Medicine-Medicine (all)

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