Rapid Identification of Jasmine Virus H Infecting Ixora coccinea by Nanopore Metatranscriptomics.

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2023-06-01 DOI:10.5423/PPJ.NT.03.2023.0037
Sung-Woong Kim, Hyo-Jeong Lee, Sena Choi, In-Sook Cho, Rae-Dong Jeong
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Abstract

The global climate change and international trade have facilitated the movement of plants across borders, increasing the risk of introducing novel plant viruses in new territories. Ixora coccinea exhibited virus-like foliar symptoms, including mosaic and mild mottle. An Oxford Nanopore Technologies-based compact and portable MinION platform was used to identify the causal viral pathogen. The complete genome sequence of jasmine virus H (JaVH; 3867 nt, JaVH-CNU) was determined and found to share 88.4-90.3% nucleotide identity with that of Jasminum sambac JaVH isolate in China. Phylogenetic analysis based on the complete amino acid sequences of RNA-dependent RNA polymerase and coat protein revealed that JaVH-CNU was grouped separately with other JaVH isolates. This is the first report of a natural JaVH infection of >i<I. coccinea. The application of rapid nanopore sequencing for plant virus identification was demonstrated and is expected to provide accurate and rapid diagnosis for virus surveillance.

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利用纳米孔超转录组学快速鉴定感染瓢虫的茉莉病毒H。
全球气候变化和国际贸易促进了植物的跨境流动,增加了在新领土引入新型植物病毒的风险。球虫表现出病毒样叶面症状,包括花叶和轻度斑驳。使用基于牛津纳米孔技术的紧凑便携式MinION平台来鉴定致病病毒病原体。茉莉病毒H (JaVH)全基因组序列;3867nt, JaVH- cnu)与中国Jasminum sambac JaVH分离株的核苷酸同源性为88.4-90.3%。基于RNA依赖性RNA聚合酶和外壳蛋白全氨基酸序列的系统发育分析表明,JaVH- cnu与其他JaVH分离株是单独类群的。这是首次报告的甲型h1n1流感自然感染病例。coccinea。快速纳米孔测序技术在植物病毒鉴定中的应用,有望为病毒监测提供准确、快速的诊断。
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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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