基于dna的合成细胞骨架工程。

IF 3.6 3区 生物学 Q1 BIOLOGY Interface Focus Pub Date : 2023-08-11 eCollection Date: 2023-10-06 DOI:10.1098/rsfs.2023.0028
Kevin Jahnke, Kerstin Göpfrich
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引用次数: 0

摘要

合成细胞与功能性细胞骨架的开发和自下而上的组装为理解细胞力学和开发纳米和微型人造机器树立了一个重要的里程碑。然而,天然的细胞骨架成分可能很难在合成细胞内纯化、精心设计和重建,因此限制了现代细胞骨架在合成细胞中实现多方面功能。在此,我们回顾了由脱氧核糖核酸(DNA)作为一种互补策略制备合成细胞骨架的最新进展。特别是,我们探索了DNA细胞骨架模拟天然膀胱骨架功能的能力和局限性,如可逆组装、货物运输、力产生、机械支撑和引导聚合。通过最近的例子,我们展示了合理设计DNA细胞骨架的力量,使自下而上组装的合成细胞成为完全可工程的实体。然而,实现动态不稳定性、自我复制和遗传编码以及产生收缩力的马达,仍然是将基于DNA的多功能细胞骨架完全整合到合成细胞中的富有成效的挑战。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Engineering DNA-based cytoskeletons for synthetic cells.

The development and bottom-up assembly of synthetic cells with a functional cytoskeleton sets a major milestone to understand cell mechanics and to develop man-made machines on the nano- and microscale. However, natural cytoskeletal components can be difficult to purify, deliberately engineer and reconstitute within synthetic cells which therefore limits the realization of multifaceted functions of modern cytoskeletons in synthetic cells. Here, we review recent progress in the development of synthetic cytoskeletons made from deoxyribonucleic acid (DNA) as a complementary strategy. In particular, we explore the capabilities and limitations of DNA cytoskeletons to mimic functions of natural cystoskeletons like reversible assembly, cargo transport, force generation, mechanical support and guided polymerization. With recent examples, we showcase the power of rationally designed DNA cytoskeletons for bottom-up assembled synthetic cells as fully engineerable entities. Nevertheless, the realization of dynamic instability, self-replication and genetic encoding as well as contractile force generating motors remains a fruitful challenge for the complete integration of multifunctional DNA-based cytoskeletons into synthetic cells.

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来源期刊
Interface Focus
Interface Focus BIOLOGY-
CiteScore
9.20
自引率
0.00%
发文量
44
审稿时长
6-12 weeks
期刊介绍: Each Interface Focus themed issue is devoted to a particular subject at the interface of the physical and life sciences. Formed of high-quality articles, they aim to facilitate cross-disciplinary research across this traditional divide by acting as a forum accessible to all. Topics may be newly emerging areas of research or dynamic aspects of more established fields. Organisers of each Interface Focus are strongly encouraged to contextualise the journal within their chosen subject.
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