{"title":"黄芪甲苷通过nmur2介导的Wnt/β-catenin通路诱导骨髓间充质干细胞向成骨细胞分化。","authors":"Yujing Cao, Qiuxia Lv, Zhihui Huang, Yang Li","doi":"10.2217/rme-2022-0184","DOIUrl":null,"url":null,"abstract":"<p><p><b>Aim:</b> Given that astragaloside-IV (As-IV) has the ability to promote osteogenic differentiation, its mechanism is worthy of exploration. <b>Methods:</b> The effect of As-IV on rat tibial defects was examined by histopathological staining and MiR-CT scan. The alkaline phosphatase (ALP) content, osteogenic differentiation-related gene expressions, and mineralized nodule formation in bone marrow mesenchymal stem cells (BMSCs) were detected. <b>Results:</b> As-IV repaired tibial defects of rats. As-IV or neuromedin receptor 2 (<i>NMUR2</i>) overexpression elevated ALP content, mineralized nodules, osteogenic differentiation-related genes, β-catenin and <i>NMUR2</i> levels, the effects of which were reversed by <i>NMUR2</i> silencing or Wnt/β-catenin pathway inhibitors. <b>Conclusion:</b> As-IV regulates the Wnt/β-catenin pathway through <i>NMUR2</i> to promote the repair of tibial defects in rats and the differentiation of BMSCs into osteoblasts.</p>","PeriodicalId":21043,"journal":{"name":"Regenerative medicine","volume":"18 6","pages":"471-485"},"PeriodicalIF":2.4000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Astragaloside-IV induces the differentiation of bone marrow mesenchymal stem cells into osteoblasts through <i>NMUR2</i>-mediated Wnt/β-catenin pathway.\",\"authors\":\"Yujing Cao, Qiuxia Lv, Zhihui Huang, Yang Li\",\"doi\":\"10.2217/rme-2022-0184\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p><b>Aim:</b> Given that astragaloside-IV (As-IV) has the ability to promote osteogenic differentiation, its mechanism is worthy of exploration. <b>Methods:</b> The effect of As-IV on rat tibial defects was examined by histopathological staining and MiR-CT scan. The alkaline phosphatase (ALP) content, osteogenic differentiation-related gene expressions, and mineralized nodule formation in bone marrow mesenchymal stem cells (BMSCs) were detected. <b>Results:</b> As-IV repaired tibial defects of rats. As-IV or neuromedin receptor 2 (<i>NMUR2</i>) overexpression elevated ALP content, mineralized nodules, osteogenic differentiation-related genes, β-catenin and <i>NMUR2</i> levels, the effects of which were reversed by <i>NMUR2</i> silencing or Wnt/β-catenin pathway inhibitors. <b>Conclusion:</b> As-IV regulates the Wnt/β-catenin pathway through <i>NMUR2</i> to promote the repair of tibial defects in rats and the differentiation of BMSCs into osteoblasts.</p>\",\"PeriodicalId\":21043,\"journal\":{\"name\":\"Regenerative medicine\",\"volume\":\"18 6\",\"pages\":\"471-485\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Regenerative medicine\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.2217/rme-2022-0184\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CELL & TISSUE ENGINEERING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Regenerative medicine","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.2217/rme-2022-0184","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL & TISSUE ENGINEERING","Score":null,"Total":0}
Astragaloside-IV induces the differentiation of bone marrow mesenchymal stem cells into osteoblasts through NMUR2-mediated Wnt/β-catenin pathway.
Aim: Given that astragaloside-IV (As-IV) has the ability to promote osteogenic differentiation, its mechanism is worthy of exploration. Methods: The effect of As-IV on rat tibial defects was examined by histopathological staining and MiR-CT scan. The alkaline phosphatase (ALP) content, osteogenic differentiation-related gene expressions, and mineralized nodule formation in bone marrow mesenchymal stem cells (BMSCs) were detected. Results: As-IV repaired tibial defects of rats. As-IV or neuromedin receptor 2 (NMUR2) overexpression elevated ALP content, mineralized nodules, osteogenic differentiation-related genes, β-catenin and NMUR2 levels, the effects of which were reversed by NMUR2 silencing or Wnt/β-catenin pathway inhibitors. Conclusion: As-IV regulates the Wnt/β-catenin pathway through NMUR2 to promote the repair of tibial defects in rats and the differentiation of BMSCs into osteoblasts.
期刊介绍：
Regenerative medicine replaces or regenerates human cells, tissue or organs, to restore or establish normal function*. Since 2006, Regenerative Medicine has been at the forefront of publishing the very best papers and reviews covering the entire regenerative medicine sector. The journal focusses on the entire spectrum of approaches to regenerative medicine, including small molecule drugs, biologics, biomaterials and tissue engineering, and cell and gene therapies – it’s all about regeneration and not a specific platform technology. The journal’s scope encompasses all aspects of the sector ranging from discovery research, through to clinical development, through to commercialization. Regenerative Medicine uniquely supports this important area of biomedical science and healthcare by providing a peer-reviewed journal totally committed to publishing the very best regenerative medicine research, clinical translation and commercialization.
Regenerative Medicine provides a specialist forum to address the important challenges and advances in regenerative medicine, delivering this essential information in concise, clear and attractive article formats – vital to a rapidly growing, multidisciplinary and increasingly time-constrained community.
Despite substantial developments in our knowledge and understanding of regeneration, the field is still in its infancy. However, progress is accelerating. The next few decades will see the discovery and development of transformative therapies for patients, and in some cases, even cures. Regenerative Medicine will continue to provide a critical overview of these advances as they progress, undergo clinical trials, and eventually become mainstream medicine.
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