鸡肌管细胞自噬的调节:线粒体氧化磷酸化的解偶联作用。

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE Journal of Poultry Science Pub Date : 2023-01-01 DOI:10.2141/jpsa.2023022
Kazuki Nakashima, Aiko Ishida
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引用次数: 0

摘要

摘要:骨骼肌对ATP的需求量很大,主要通过线粒体氧化磷酸化来满足。在分解代谢条件下,自噬对维持骨骼肌质量至关重要。本研究探讨了脱偶联线粒体氧化磷酸化对鸡骨骼肌自噬的影响。用线粒体解偶联剂羰基氰间氯苯腙(CCCP)在25 μM下孵育鸡肌管3h。CCCP阻断了p70核糖体S6激酶1 (Thr389)、S6核糖体蛋白(Ser240/244)和真核翻译起始因子4e结合蛋白1 (Thr37/46)的磷酸化,这些磷酸化是雷帕霉素复合物1 (mTORC1)活性的机制靶点。CCCP显著提高了细胞质和线粒体LC3-II含量,LC3-II分别是自噬体形成和自噬的指标,但不影响自噬相关基因LC3B、GABARAPL1和ATG12的表达。最后,翻译方法的表面传感显示,CCCP处理显著降低了蛋白质合成这一高能量消耗过程。这些结果表明,在鸡肌管培养中,线粒体氧化磷酸化的解偶联刺激自噬并通过mTORC1信号抑制蛋白质合成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Regulation of autophagy in chick myotube cultures: Effect of uncoupling mitochondrial oxidative phosphorylation.

Abstracts: Skeletal muscles have a high demand for ATP, which is met largely through mitochondria oxidative phosphorylation. Autophagy is essential for the maintenance of skeletal muscle mass under catabolic conditions. This study investigated the effect of uncoupling mitochondrial oxidative phosphorylation on autophagy in chicken skeletal muscle. Chick myotubes were incubated with the mitochondrial uncoupler carbonyl cyanide m-chlorophenyl hydrazone (CCCP) at 25 μM for 3h. CCCP prevented the phosphorylation of p70 ribosomal S6 kinase 1 (Thr389), S6 ribosomal protein (Ser240/244), and eukaryotic translation initiation factor 4E-binding protein 1 (Thr37/46), which are the measures of the mechanistic target of rapamycin complex 1 (mTORC1) activity. CCCP significantly increased cytoplasmic and mitochondrial LC3-II content, which act as indices of index for autophagosome formation and mitophagy, respectively, but did not influence the expression of autophagy-related genes LC3B, GABARAPL1, and ATG12. Finally, surface sensing of translation method revealed that protein synthesis, a highly energy consuming process, was significantly decreased upon CCCP treatment. These results indicate that the uncoupling of mitochondrial oxidative phosphorylation stimulates autophagy and inhibits protein synthesis through mTORC1 signaling in chick myotube cultures.

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来源期刊
Journal of Poultry Science
Journal of Poultry Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
2.80
自引率
13.30%
发文量
26
审稿时长
12 months
期刊介绍: The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.
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