顺式调控元件的不同组合控制表型可塑性的进化。

IF 7.8 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY PLoS Biology Pub Date : 2023-08-17 eCollection Date: 2023-08-01 DOI:10.1371/journal.pbio.3002270
Mohannad Dardiry, Gabi Eberhard, Hanh Witte, Christian Rödelsperger, James W Lightfoot, Ralf J Sommer
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引用次数: 1

摘要

表型可塑性在生命各个领域的广泛存在证明了其进化意义。然而,可塑性本身是如何进化的,以及它是如何促进进化的,人们对此知之甚少。在此,我们利用重组自交系和天然分离株之间的数量性状位点(QTL)分析,研究了捕食性线虫和平原(Prisionchus pacificus)及其取食结构的可塑性。我们发现,核心发育基因上的单个QTL控制着同类相食变体的表达。该QTL由几个顺式调控元件组成。通过CRISPR/Cas-9工程,我们确定了与单个内含子核苷酸多态性相互作用的潜在转录因子结合位点的拷贝数变化。另一个内含子元素完全消除了基因表达,模仿基因座的敲除。额外分离物的比较进一步支持了这些顺式调节元件的快速进化。最后,一项独立的QTL研究揭示了在同一基因座平行进化的证据。因此,顺式调控元件的组合塑造了可塑性性状的表达并控制了线虫的自相残杀。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Divergent combinations of cis-regulatory elements control the evolution of phenotypic plasticity.

The widespread occurrence of phenotypic plasticity across all domains of life demonstrates its evolutionary significance. However, how plasticity itself evolves and how it contributes to evolution is poorly understood. Here, we investigate the predatory nematode Pristionchus pacificus with its feeding structure plasticity using recombinant-inbred-line and quantitative-trait-locus (QTL) analyses between natural isolates. We show that a single QTL at a core developmental gene controls the expression of the cannibalistic morph. This QTL is composed of several cis-regulatory elements. Through CRISPR/Cas-9 engineering, we identify copy number variation of potential transcription factor binding sites that interacts with a single intronic nucleotide polymorphism. Another intronic element eliminates gene expression altogether, mimicking knockouts of the locus. Comparisons of additional isolates further support the rapid evolution of these cis-regulatory elements. Finally, an independent QTL study reveals evidence for parallel evolution at the same locus. Thus, combinations of cis-regulatory elements shape plastic trait expression and control nematode cannibalism.

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来源期刊
PLoS Biology
PLoS Biology 生物-生化与分子生物学
CiteScore
14.40
自引率
2.00%
发文量
359
审稿时长
3 months
期刊介绍: PLOS Biology is an open-access, peer-reviewed general biology journal published by PLOS, a nonprofit organization of scientists and physicians dedicated to making the world's scientific and medical literature freely accessible. The journal publishes new articles online weekly, with issues compiled and published monthly. ISSN Numbers: eISSN: 1545-7885 ISSN: 1544-9173
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