Virginia Quinan, Kelly Hsu, Molly Mann, Kia Barclay, Deborah Bauer
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引用次数: 0
摘要
本科生神经科学实验室为学生通过主动学习了解神经生物学系统提供了宝贵的机会。秀丽隐杆线虫(C. elegans)是教授学生如何使用还原论方法进行神经科学探究的宝贵模型。这一系列实验模块训练学生使用基础实验技术,如蠕虫处理和维护、荧光成像、行为测定和 Western 印迹。在完成这一系列实验练习后,学生就能为使用这些研究技术参与独立研究项目做好充分准备。学生调查结果表明,这一系列的 elegans 实验练习培养了学生的基本研究技能,学生可以将这些技能应用到未来的各种科学研究中。
An Undergraduate Laboratory Series Using C. elegans That Prepares Students for Independent Inquiry.
Undergraduate neuroscience laboratories provide valuable opportunities for students to learn about neurobiological systems through active learning. Caenorhabditis elegans (C. elegans) is a valuable model for teaching students how to use a reductionist approach to neuroscientific inquiry. This series of lab modules trains students to utilize foundational laboratory techniques such as worm handling and maintenance, fluorescence imaging, behavioral assays, and Western blot. Upon completing this series of laboratory exercises, students are well prepared to engage in independent research projects using these research techniques. As supported by student survey results, this series of C. elegans laboratory exercises leads to the development of essential research skills, which students may be able to apply to a wide range of future scientific endeavors.