用于改进生物技术相关生物散射光测量的3d打印光学修饰培养容器的研究和评估

IF 3.9 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Engineering in Life Sciences Pub Date : 2023-08-23 DOI:10.1002/elsc.202300204
Johanna S. Rehfeld, Louis M. Kuhnke, Christian Ude, Gernot T. John, Sascha Beutel
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引用次数: 1

摘要

在生物过程开发领域,小型化、并行化和灵活性在降低成本和时间方面发挥着关键作用。为了精确满足这些要求,增材制造(3D打印)是一种理想的技术。3D打印能够根据需要快速成型并经济高效地制造具有复杂几何形状的单独设计的设备。为了成功开发生物工艺,监测工艺相关参数,如pH、溶解氧(DO)和生物量,至关重要。由于离线采样耗时且会导致信息丢失,因此首选在线监测。在这项研究中,评估了具有光学棱镜的3D打印培养容器在不同工业相关微生物和细胞系的上游过程中的用途。研究表明,3D打印的光学修饰井(OMW)对各种生物技术相关的微生物,甚至对哺乳动物悬浮细胞都有好处。用大肠杆菌、枯草芽孢杆菌、酿酒酵母和中国仓鼠卵巢(CHO)细胞进行了评估测试,提供了高度可重复的结果。OMW培养物的生长行为与摇瓶(SF)培养物的行为相当,并且通过使用OMW,在线生物量测量中的信噪比降低了95.8%。特别是在浊度较低的培养阶段,即光密度低于1.0 rel.AU的培养阶段可以首次得到准确的监测。此外,研究表明,3D打印的光学器件可以转移到不同的井几何形状和尺寸,通过小规模定制的3D打印培养容器,能够有效监测个人需求的生物量。
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Investigation and evaluation of a 3D-printed optical modified cultivation vessel for improved scattered light measurement of biotechnologically relevant organisms

In the field of bioprocess development miniaturization, parallelization and flexibility play a key role reducing costs and time. To precisely meet these requirements, additive manufacturing (3D-printing) is an ideal technology. 3D-printing enables rapid prototyping and cost-effective fabrication of individually designed devices with complex geometries on demand. For successful bioprocess development, monitoring of process-relevant parameters, such as pH, dissolved oxygen (DO), and biomass, is crucial. Online monitoring is preferred as offline sampling is time-consuming and leads to loss of information. In this study, 3D-printed cultivation vessels with optical prisms are evaluated for the use in upstream processes of different industrially relevant microorganisms and cell lines. It was shown, that the 3D-printed optically modified well (OMW) is of benefit for a wide range of biotechnologically relevant microorganisms and even for mammalian suspension cells. Evaluation tests with Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae, and Chinese hamster ovary (CHO) cells were performed, providing highly reproducible results. Growth behavior of OMW cultures was comparable to behavior of shake flask (SF) cultivations and the signal to noise ratio in online biomass measurement was shown to be reduced up to 95.8% by using the OMW. Especially the cultivation phases with low turbidity respective optical densities below 1.0 rel.AU could be monitored accurately for the first time. Furthermore, it was demonstrated that the 3D-printed optics are transferable to different well geometries and sizes, enabling efficient biomass monitoring for individual requirements with tailor-made 3D-printed cultivation vessels in small scale.

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来源期刊
Engineering in Life Sciences
Engineering in Life Sciences 工程技术-生物工程与应用微生物
CiteScore
6.40
自引率
3.70%
发文量
81
审稿时长
3 months
期刊介绍: Engineering in Life Sciences (ELS) focuses on engineering principles and innovations in life sciences and biotechnology. Life sciences and biotechnology covered in ELS encompass the use of biomolecules (e.g. proteins/enzymes), cells (microbial, plant and mammalian origins) and biomaterials for biosynthesis, biotransformation, cell-based treatment and bio-based solutions in industrial and pharmaceutical biotechnologies as well as in biomedicine. ELS especially aims to promote interdisciplinary collaborations among biologists, biotechnologists and engineers for quantitative understanding and holistic engineering (design-built-test) of biological parts and processes in the different application areas.
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