邻苯二甲酸酯和双酚增塑剂对草地贪夜蛾糖酵解酶活性的影响

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY Journal of insect physiology Pub Date : 2023-09-01 DOI:10.1016/j.jinsphys.2023.104533
Johanna Rivas , Annabelle Fuentes , Annick Maria , Benjamin Bergerot , David Siaussat , David Renault
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引用次数: 0

摘要

近几十年来,环境塑料污染显著增加,严重影响了经济、人类和生物多样性健康。塑料由几种化学添加剂制成,包括双酚A和邻苯二甲酸酯增塑剂,如双酚A和邻苯二甲酸二(2-乙基己基)酯(DEHP)。在一些动物物种中,BPA和DEHP都被称为内分泌干扰物,可以改变生理和代谢稳态、繁殖、发育和/或行为。到目前为止,BPA和DEHP的影响主要集中在脊椎动物身上,对水生无脊椎动物的影响较小。然而,为数不多的研究检测了DEHP对陆地昆虫的影响,也揭示了这种污染物对发育、激素滴定和代谢谱的影响。特别是,在埃及棉叶虫Spodoptera littoralis中,假设观察到的代谢变化可能是由于DEHP解毒所需的能量成本或激素控制的酶活性失调造成的。为了进一步深入了解双酚A和邻苯二甲酸酯增塑剂对石蛾的生理影响,用被BPA、DEHP或这两种化合物的混合物污染的食物喂养幼虫。然后,测定了己糖激酶、磷酸葡萄糖异构酶、磷酸果糖激酶和丙酮酸激酶四种糖酵解酶的活性。BPA和/或DEHP对磷酸果糖激酶和丙酮酸激酶活性无影响。相反,BPA污染的幼虫的特征是磷酸葡萄糖异构酶活性增加了1.9倍,而BPA+DEHP喂养的幼虫具有高度可变的己糖激酶活性。总的来说,由于在DEHP污染的幼虫中没有观察到糖酵解酶的破坏,我们的工作倾向于证明暴露于双酚和DEHP会增加所经历的氧化应激量。
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Effects of phthalate and bisphenol plasticizers on the activity of glycolytic enzymes of the moth Spodoptera littoralis

Environmental plastic pollution has significantly increased in the recent decades, and severely impacts economies, human and biodiversity health. Plastics are made of several chemical additives, including bisphenol and phthalate plasticizers such as bisphenol A (BPA) and Di(2-ethylhexyl)phthalate (DEHP). In some animal species, both BPA and DEHP are known as endocrine disruptor compounds, and can alter physiological and metabolic homeostasis, reproduction, development and/or behavior. To date, the impacts of BPA and DEHP have mainly focused on vertebrates, and to a lesser extent, on aquatic invertebrates. Yet, the few studies which examined the effects of DEHP on terrestrial insects also revealed the impacts this pollutant can have on development, hormone titrations, and metabolic profiles. In particular, it has been hypothesized in the Egyptian cotton leafworm Spodoptera littoralis that the observed metabolic alterations could result from the energetic costs necessary for DEHP detoxification or to the dysregulation of hormonally-controlled enzymatic activities. To get additional insights into the physiological effects of bisphenol and phthalate plasticizers on the moth S. littoralis, larvae were fed with food contaminated by BPA, DEHP, or the mixture of both compounds. Then, activities of four glycolytic enzymes, hexokinase, phosphoglucose isomerase, phosphofructokinase, and pyruvate kinase were measured. BPA and/or DEHP had no effects on the activities of phosphofructokinase and pyruvate kinase. Conversely, BPA-contaminated larvae were characterized by a 1.9-fold increase in phosphoglucose isomerase activity, and BPA + DEHP-fed larvae had highly variable hexokinase activity. Overall, since no disruption of glycolytic enzyme was observed in DEHP-contaminated larvae, our work tended to demonstrate that exposure to bisphenol and DEHP increased the amount of oxidative stress experienced.

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来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
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