Christina L Elling, Salina H Goff, Scott D Hirsch, Kaitlyn Tholen, Jennifer M Kofonow, Danielle Curtis, Charles E Robertson, Jeremy D Prager, Patricia J Yoon, Todd M Wine, Kenny H Chan, Melissa A Scholes, Norman R Friedman, Daniel N Frank, Brian W Herrmann, Regie Lyn P Santos-Cortez
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引用次数: 0
摘要
背景:中耳炎(OM)是指通常由感染引起的中耳(ME)炎症。在全球范围内,中耳炎是导致听力损失的主要原因,也是幼儿最常诊断出的疾病。就中耳炎而言,患有唐氏综合征(DS)的儿童患者发病率更高、病情更严重、治疗效果更差。然而,迄今为止,还没有研究对患有唐氏综合征和 OM 的儿童的细菌谱进行调查。方法:我们的目的是确定患有 DS 的小儿 OM 患者(n = 11)与不患有 DS 的小儿 OM 患者(n = 84)相比,其 ME 和鼻咽 (NP) 微生物群中的细菌谱组成或单个类群的相对丰度是否存在差异。我们对 16S rRNA 基因进行了测序,并分析了序列数据的多样性指数和各个类群的相对丰度。结果显示DS 患儿的 ME 和 NP 微生物群的生物多样性有所增加。在 OM 患儿中,DS 与 ME 中生物多样性增加和特定类群相对丰度较高有关。结论:我们的研究结果表明,与对照组相比,DS 儿童 NP 中的菌群失调是导致他们更易患 OM 的原因之一。这些研究结果表明,DS会影响粘膜微生物群的调节,并导致OM病理变化。
Otitis Media in Children with Down Syndrome Is Associated with Shifts in the Nasopharyngeal and Middle Ear Microbiotas.
Background: Otitis media (OM) is defined as middle ear (ME) inflammation that is usually due to infection. Globally, OM is a leading cause of hearing loss and is the most frequently diagnosed disease in young children. For OM, pediatric patients with Down syndrome (DS) demonstrate higher incidence rates, greater severity, and poorer outcomes. However, to date, no studies have investigated the bacterial profiles of children with DS and OM. Method: We aimed to determine if there are differences in composition of bacterial profiles or the relative abundance of individual taxa within the ME and nasopharyngeal (NP) microbiotas of pediatric OM patients with DS (n = 11) compared with those without DS (n = 84). We sequenced the 16S rRNA genes and analyzed the sequence data for diversity indices and relative abundance of individual taxa. Results: Individuals with DS demonstrated increased biodiversity in their ME and NP microbiotas. In children with OM, DS was associated with increased biodiversity and higher relative abundance of specific taxa in the ME. Conclusion: Our findings suggest that dysbioses in the NP of DS children contributes to their increased susceptibility to OM compared with controls. These findings suggest that DS influences regulation of the mucosal microbiota and contributes to OM pathology.
期刊介绍:
Genetic Testing and Molecular Biomarkers is the leading peer-reviewed journal covering all aspects of human genetic testing including molecular biomarkers. The Journal provides a forum for the development of new technology; the application of testing to decision making in an increasingly varied set of clinical situations; ethical, legal, social, and economic aspects of genetic testing; and issues concerning effective genetic counseling. This is the definitive resource for researchers, clinicians, and scientists who develop, perform, and interpret genetic tests and their results.
Genetic Testing and Molecular Biomarkers coverage includes:
-Diagnosis across the life span-
Risk assessment-
Carrier detection in individuals, couples, and populations-
Novel methods and new instrumentation for genetic testing-
Results of molecular, biochemical, and cytogenetic testing-
Genetic counseling