Siyuan Li, Diyang Chen, Huamin Zhang, Yong Yang, Jianguo Huai, Linna Huang, Kai Fan, Tongyuan Lin, Baijing Ding
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We analyzed relationships between ZNF471 expression levels and characteristics, such as tumor size, gender, age, TNM stage, and lymph node metastasis.</p><p><strong>Results: </strong>Immunohistochemistry revealed the expression of ZNF471 protein from paraffin blocks of GC tissues was significantly lower than that of adjacent tissues. Expression levels of ZNF471 mRNA and protein in fresh GC tissues were markedly lower than those in adjacent tissues and in normal gastric mucosal tissues from healthy subjects. ZNF471 expression was significantly correlated with tumor size, lymph node metastasis, and TNM stage (all <i>P</i><0.05). There were no significant associations with gender, age, distant metastasis, or pathologic type. Expression of ZNF471 mRNA and protein was not significantly different between adjacent tissues of patients with GC and normal gastric mucosal tissue from healthy subjects.</p><p><strong>Conclusion: </strong>ZNF471 functions as a tumor suppressor during the pathogenesis of GC. Thus, it is a promising biomarker for diagnosis and therapy of GC.</p>","PeriodicalId":13943,"journal":{"name":"International journal of clinical and experimental pathology","volume":"16 8","pages":"199-208"},"PeriodicalIF":1.1000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10492033/pdf/ijcep0016-0199.pdf","citationCount":"0","resultStr":"{\"title\":\"Clinical significance of expression level of ZNF471 in gastric cancer.\",\"authors\":\"Siyuan Li, Diyang Chen, Huamin Zhang, Yong Yang, Jianguo Huai, Linna Huang, Kai Fan, Tongyuan Lin, Baijing Ding\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>As a tumor suppressor gene, zinc finger protein 471 (ZNF471) has an essential role in tumor occurrence and development. Due to promoter hypermethylation, it can be underexpressed or silenced in gastric cancer (GC) cell lines. 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引用次数: 0
摘要
背景:锌指蛋白471 (ZNF471)作为一种肿瘤抑制基因,在肿瘤的发生发展中起着至关重要的作用。由于启动子的高甲基化,它在胃癌细胞系中可以低表达或沉默。在本研究中,我们探讨了GC患者组织中ZNF471表达水平与临床特征的关系。方法:采用免疫组化(IHC)法检测石蜡组织标本中ZNF471的表达,采用实时荧光定量PCR (qRT-PCR)和western blot (WB)法检测新鲜组织标本中ZNF471的表达水平。我们分析了ZNF471表达水平与肿瘤大小、性别、年龄、TNM分期和淋巴结转移等特征的关系。结果:免疫组化显示GC组织石蜡块中ZNF471蛋白的表达明显低于邻近组织。新鲜胃粘膜组织中ZNF471 mRNA和蛋白的表达水平明显低于邻近组织和健康人正常胃粘膜组织。ZNF471的表达与肿瘤大小、淋巴结转移、TNM分期均有显著相关性(均为p)。结论:ZNF471在胃癌发病过程中发挥抑癌作用。因此,它是一种有前景的生物标志物,用于胃癌的诊断和治疗。
Clinical significance of expression level of ZNF471 in gastric cancer.
Background: As a tumor suppressor gene, zinc finger protein 471 (ZNF471) has an essential role in tumor occurrence and development. Due to promoter hypermethylation, it can be underexpressed or silenced in gastric cancer (GC) cell lines. In this study, we investigated relationships between clinical characteristics and ZNF471 expression levels in tissues of patients with GC.
Methods: We used immunohistochemistry (IHC) to detect ZNF471 expression in paraffin tissue specimens, and quantitative real-time PCR (qRT-PCR) and western blot (WB) analysis to measure expression levels of ZNF471 in fresh tissue specimens. We analyzed relationships between ZNF471 expression levels and characteristics, such as tumor size, gender, age, TNM stage, and lymph node metastasis.
Results: Immunohistochemistry revealed the expression of ZNF471 protein from paraffin blocks of GC tissues was significantly lower than that of adjacent tissues. Expression levels of ZNF471 mRNA and protein in fresh GC tissues were markedly lower than those in adjacent tissues and in normal gastric mucosal tissues from healthy subjects. ZNF471 expression was significantly correlated with tumor size, lymph node metastasis, and TNM stage (all P<0.05). There were no significant associations with gender, age, distant metastasis, or pathologic type. Expression of ZNF471 mRNA and protein was not significantly different between adjacent tissues of patients with GC and normal gastric mucosal tissue from healthy subjects.
Conclusion: ZNF471 functions as a tumor suppressor during the pathogenesis of GC. Thus, it is a promising biomarker for diagnosis and therapy of GC.
期刊介绍:
The International Journal of Clinical and Experimental Pathology (IJCEP, ISSN 1936-2625) is a peer reviewed, open access online journal. It was founded in 2008 by an international group of academic pathologists and scientists who are devoted to the scientific exploration of human disease and the rapid dissemination of original data. Unlike most other open access online journals, IJCEP will keep all the traditional features of paper print that we are all familiar with, such as continuous volume and issue numbers, as well as continuous page numbers to keep our warm feelings towards an academic journal. Unlike most other open access online journals, IJCEP will keep all the traditional features of paper print that we are all familiar with, such as continuous volume and issue numbers, as well as continuous page numbers to keep our warm feelings towards an academic journal.