Maria C Vercellini, Ramiro Rearte, Luca di Cesare, Miguel A Ayala, Martin M Montes
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引用次数: 0
摘要
在水产养殖和实验过程中,麻醉剂通常用于鱼类的外科手术以及便于捕获、处理和运输。在研究中,麻醉剂的选择取决于其特性和恢复时间。有人指出,丁香酚是一种有效的鱼类麻醉剂,可替代传统药物。虽然 Cnesterodon decemmaculatus 被广泛用作生态生物测定的模型,但还没有关于该物种麻醉方案的报道。本研究的目的是根据鱼的性别和怀孕状况,评估鱼类个体在固定浓度丁香酚作用下的诱导时间(即达到麻醉阶段 VI 的时间)和恢复时间。41 条鱼被分为三组:雄鱼、怀孕雌鱼和未怀孕雌鱼。测量它们的总长度、标准长度和体重,并计算条件因子(K)。各组之间的诱导和恢复时间在性别、怀孕状态和 K 方面均无明显差异。研究结果有助于为栉水母制定标准麻醉方案。
Eugenol as anesthetic for Cnesterodon decemmaculatus (Cyprinodontiformes, Poeciliidae).
Anesthetics are commonly used in fish for surgery and to facilitate capture, handling and transport in aquaculture and experimental procedures. In research, the selection of the anesthetic depends on its properties and on the recovery time. Eugenol has been pointed out as an effective anesthetic for fish, alternative to traditional drugs. Although Cnesterodon decemmaculatus is widely used as a model in ecological bioassays, no anesthetic protocol has been reported for this species. The aim of the present study was to evaluate the induction time (i.e. time to reach anesthetic stage VI) and recovery time in individuals of C. decemmaculatus subjected to eugenol at a fixed concentration, according to sex and pregnancy status. Forty-one fish were divided into three groups: males, pregnant females and non-pregnant females. They were measured for total length, standard length and weight, and the condition factor (K) was calculated. No significant differences in induction and recovery times were found for sex, pregnancy status and K between groups. Results are a contribution toward the development protocol of a standard anesthetic protocol for C. decemmaculatus.
期刊介绍:
The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.