监测野生鸟类和牛中某些大肠杆菌菌株的遗传变异。

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES Onderstepoort Journal of Veterinary Research Pub Date : 2023-07-26 DOI:10.4102/ojvr.v90i1.2085
Ghada A Ibrahim, Ahmed M Salah-Eldein, Mayasar I Al-Zaban, Amal S A El-Oksh, Elsayyad M Ahmed, Doaa S Farid, Enas M Saad
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摘要

迄今为止,关于野生鸟类和牛之间大肠杆菌遗传关系的数据有限,因为这些鸟类是许多人畜共患细菌的沉默载体。本研究旨在阐明在牛场附近饲养的野生鸟类在相同致病性大肠杆菌变异传播中的作用,鉴定它们的毒力、抗性性状和fimH毒力基因的遗传相似性。收集了约240个粪/肛肠拭子,并进行了细菌学检查。大肠杆菌的检出率分别为45.8%和32.5%。检出最多的大肠杆菌血清型为O26。四环素、氯霉素耐药高;而庆大霉素和环丙沙星的敏感性最高。详细讨论了大肠杆菌分离株的聚合酶链反应保守型耐药性(tetA和blaCTX-M)和毒力特性(fimH、stx1、eaeA和ompA)。fimH基因在全球和本地不同大肠杆菌分离株的序列相似性为100%。最后,检测到大肠杆菌与野生鸟类和牛的密切遗传关联,从而加强了其在通过环境传播感染中的作用。由于大肠杆菌对牲畜和动物工作者构成巨大的人畜共患风险,应采取预防和保守政策。此外,建议对大肠杆菌的fimH、其他毒力和耐药基因的全基因组测序进行进一步研究,以限制不同物种间共感染和转移的可能性。贡献:目前的研究记录了有关野生鸟类对牲畜(包括埃及的养牛场)的关键感染作用的最新数据。它还就牛场处理动物粪便时必须实施的良好卫生规范提出了一些建议。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Monitoring the genetic variation of some Escherichia coli strains in wild birds and cattle.

To date, there is limited data about the genetic relationship of Escherichia coli between wild birds and cattle because these birds act as silent vectors for many zoonotic bacteria. This study aimed to elucidate the role of rooming wild birds in the vicinity of cattle farm in transmission of the same pathogenic E. coli variants, identifying their virulence, resistance traits and genetic similarities of fimH virulence gene. About 240 faecal/cloacal swabs were collected from both species and examined bacteriologically. Escherichia coli was yielded in 45.8% and 32.5%, respectively, of examined cattle and wild birds. The most prevalent detected E. coli serovar was O26. High tetracycline and chloramphenicol resistance were recorded; however, gentamycin and ciprofloxacin exhibited the highest sensitivity rates. Polymerase chain reaction (PCR) conserved genotypic resistance (tetA and blaCTX-M) and virulence attributes (fimH, stx1, eaeA and ompA) of E. coli isolates were discussed in detail. The fimH gene revealed 100% sequence similarity when comparing with different E. coli isolates globally and locally. Finally, a close genetic association of E. coli with both wild birds and cattle was detected, thus strengthening its role in the dissemination of the infection via environment. Prevention and conservative policy should be carried as E. coli constitute enormous significant zoonotic risks to livestock and animal workers. Also, further studies to the whole genome sequencing of fimH, other virulence and resistance genes of E. coli are recommended trying to limit the possibilities of co-infection and transfer among different species.Contribution: The current study recorded updated data about the critical infectious role of wild birds to livestock, including cattle farms in Egypt. It also delivered some recommendations for good hygienic practices in cattle farms which must be implemented for handling animal manure.

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来源期刊
CiteScore
4.30
自引率
0.00%
发文量
13
审稿时长
16 weeks
期刊介绍: The Onderstepoort Journal of Veterinary Research, is the official publication of the Onderstepoort Veterinary Institute. While it considers submissions from any geographic region, its focus is on Africa and the infectious and parasitic diseases and disease vectors that affect livestock and wildlife on the continent.
期刊最新文献
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