芽孢杆菌pfraonensis sp.nov.,一株从益生菌饲料添加剂中分离得到的具有低细胞毒性和抗菌活性的新菌株。

IF 2.5 4区 生物学 Q3 MICROBIOLOGY Research in microbiology Pub Date : 2023-09-01 DOI:10.1016/j.resmic.2023.104090
Fengru Deng , Yunsheng Chen , Huiying Xiao , Chuying Yao , Jiaqi Chen , Yuanting Zhao , Yapei Jiang , Jiahang Chen , Yiqun Deng , Jikai Wen
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引用次数: 0

摘要

含有活微生物的益生菌产品越来越受欢迎,增加了其分类地位的重要性。由益生菌饲料添加剂培养的芽孢杆菌样分离物70b在分类上存在歧义,可能是蜡样芽孢杆菌群的新成员。菌落和细胞形态、主要包括生长性能、碳源利用以及rMLST和MLST的生理生化分析结果均不具有结论性。脂肪酸图谱和分子遗传学分析,特别是ANI、DDH和基于核心基因组SNPs的系统发育树,证实70b是蜡样芽孢杆菌属的一个新种,并被认为是pfraonensis sp.nov。比较基因组分析揭示了70b与蜡样芽孢菌属其他物种的遗传差异。假霉菌毒素在70 b中被鉴定。70b对耐多药致病菌株MRSA具有活性。研究结果支持70b是一种具有低细胞毒性和抗微生物活性的新物种,并提供了对其独特特性和益生菌潜力的更好理解,以及对生物活性潜力的探索。
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Bacillus pfraonensis sp. nov., a new strain isolated from a probiotic feed additive with low cytotoxicity and antimicrobial activity

Probiotic products containing living microorganisms are gaining popularity, increasing the importance of their taxonomic status. A Bacillus-like isolate, 70 b, cultured from a probiotic feed additive, was ambiguity in taxonomic assignment and could be a novel member of Bacillus cereus group. The results of colony and cellular morphology, physiological and biochemical analysis mainly including growth performance, carbon source utilization, and rMLST and MLST were not conclusive. Fatty acids profile and molecular genetic analysis especially ANI, DDH, and core genome SNPs-based phylogenetic tree confirmed 70 b as one novel species of B. cereus group and proposed as Bacillus pfraonensis sp. nov. Comparative genomic analysis revealed the genetic differences between 70 b and other species of B. cereus group. Pseudomycoicidin was identified in 70 b. 70 b was active against multidrug-resistant pathogenic strains MRSA. The findings support 70 b is a novel species with low cytotoxicity and antimicrobial activity, and provides a better understanding of its unique characteristics and probiotic potential, and exploration of bioactive potential.

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来源期刊
Research in microbiology
Research in microbiology 生物-微生物学
CiteScore
4.10
自引率
3.80%
发文量
54
审稿时长
16 days
期刊介绍: Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.
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