Junkai Zhang , Yingying Yang , Huarun Sun , Xingwei Luo , Xiaodie Cui , Qingqing Miao , Dandan He , Jinfeng Zhao , Fengbin Yan , Yushan Pan , Yajun Zhai , Gongzheng Hu
{"title":"optrA基因在猪链球菌病猪分离株中的患病率和一种新型整合偶联元件ICESsu988S的鉴定。","authors":"Junkai Zhang , Yingying Yang , Huarun Sun , Xingwei Luo , Xiaodie Cui , Qingqing Miao , Dandan He , Jinfeng Zhao , Fengbin Yan , Yushan Pan , Yajun Zhai , Gongzheng Hu","doi":"10.1016/j.resmic.2023.104078","DOIUrl":null,"url":null,"abstract":"<div><p>Aim of this study was to investigate the prevalence and genetic environment of the oxazolidinone resistance gene <em>optrA</em> in <span><em>Streptococcus suis</em></span> (<em>S. suis</em>) isolates from diseased pigs in China. A total of 178 <em>S. suis</em> isolates were screened for the <em>optrA</em> gene by PCR. The phenotypes and genotypes of <em>optrA</em><span>-positive isolates were investigated by antimicrobial susceptibility<span><span> testing, core genome Multilocus Sequence Typing (cgMLST), capsular </span>serotypes determination and whole-genome sequencing (WGS). Fifty-one (28.7%) </span></span><em>S. suis</em> isolates were positive for <em>optrA</em><span>. Phylogenetic analysis indicated that the spread of the </span><em>optrA</em> among <em>S. suis</em> isolates was primarily due to horizontal transfer. Analysis of <em>S. suis</em> serotypes from diseased pigs revealed substantial diversity. The genetic environment of <em>optrA</em> was complex and diverse and could be divided into 12 different types. Interestingly, we identified a novel integrative and conjugative element ICE<em>Ssu</em>988S, carrying <em>optrA</em> and <em>erm</em>(T) genes. This is to the best of our knowledge the first report of the <em>optrA</em> and <em>erm</em>(T) co-located on an ICE in <em>S. suis</em>. Our results showed a high prevalence of <em>optrA</em> gene in <em>S. suis</em> isolates in China. Further research is needed to evaluate the importance of ICEs, as they horizontally propagate important clinical resistance genes.</p></div>","PeriodicalId":21098,"journal":{"name":"Research in microbiology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Prevalence of the optrA gene among Streptococcus suis isolates from diseased pigs and identification of a novel integrative conjugative element ICESsu988S\",\"authors\":\"Junkai Zhang , Yingying Yang , Huarun Sun , Xingwei Luo , Xiaodie Cui , Qingqing Miao , Dandan He , Jinfeng Zhao , Fengbin Yan , Yushan Pan , Yajun Zhai , Gongzheng Hu\",\"doi\":\"10.1016/j.resmic.2023.104078\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Aim of this study was to investigate the prevalence and genetic environment of the oxazolidinone resistance gene <em>optrA</em> in <span><em>Streptococcus suis</em></span> (<em>S. suis</em>) isolates from diseased pigs in China. A total of 178 <em>S. suis</em> isolates were screened for the <em>optrA</em> gene by PCR. The phenotypes and genotypes of <em>optrA</em><span>-positive isolates were investigated by antimicrobial susceptibility<span><span> testing, core genome Multilocus Sequence Typing (cgMLST), capsular </span>serotypes determination and whole-genome sequencing (WGS). Fifty-one (28.7%) </span></span><em>S. suis</em> isolates were positive for <em>optrA</em><span>. Phylogenetic analysis indicated that the spread of the </span><em>optrA</em> among <em>S. suis</em> isolates was primarily due to horizontal transfer. Analysis of <em>S. suis</em> serotypes from diseased pigs revealed substantial diversity. The genetic environment of <em>optrA</em> was complex and diverse and could be divided into 12 different types. Interestingly, we identified a novel integrative and conjugative element ICE<em>Ssu</em>988S, carrying <em>optrA</em> and <em>erm</em>(T) genes. This is to the best of our knowledge the first report of the <em>optrA</em> and <em>erm</em>(T) co-located on an ICE in <em>S. suis</em>. Our results showed a high prevalence of <em>optrA</em> gene in <em>S. suis</em> isolates in China. Further research is needed to evaluate the importance of ICEs, as they horizontally propagate important clinical resistance genes.</p></div>\",\"PeriodicalId\":21098,\"journal\":{\"name\":\"Research in microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Research in microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0923250823000530\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Research in microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0923250823000530","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Prevalence of the optrA gene among Streptococcus suis isolates from diseased pigs and identification of a novel integrative conjugative element ICESsu988S
Aim of this study was to investigate the prevalence and genetic environment of the oxazolidinone resistance gene optrA in Streptococcus suis (S. suis) isolates from diseased pigs in China. A total of 178 S. suis isolates were screened for the optrA gene by PCR. The phenotypes and genotypes of optrA-positive isolates were investigated by antimicrobial susceptibility testing, core genome Multilocus Sequence Typing (cgMLST), capsular serotypes determination and whole-genome sequencing (WGS). Fifty-one (28.7%) S. suis isolates were positive for optrA. Phylogenetic analysis indicated that the spread of the optrA among S. suis isolates was primarily due to horizontal transfer. Analysis of S. suis serotypes from diseased pigs revealed substantial diversity. The genetic environment of optrA was complex and diverse and could be divided into 12 different types. Interestingly, we identified a novel integrative and conjugative element ICESsu988S, carrying optrA and erm(T) genes. This is to the best of our knowledge the first report of the optrA and erm(T) co-located on an ICE in S. suis. Our results showed a high prevalence of optrA gene in S. suis isolates in China. Further research is needed to evaluate the importance of ICEs, as they horizontally propagate important clinical resistance genes.
期刊介绍：
Research in Microbiology is the direct descendant of the original Pasteur periodical entitled Annales de l''Institut Pasteur, created in 1887 by Emile Duclaux under the patronage of Louis Pasteur. The Editorial Committee included Chamberland, Grancher, Nocard, Roux and Straus, and the first issue began with Louis Pasteur''s "Lettre sur la Rage" which clearly defines the spirit of the journal:"You have informed me, my dear Duclaux, that you intend to start a monthly collection of articles entitled "Annales de l''Institut Pasteur". You will be rendering a service that will be appreciated by the ever increasing number of young scientists who are attracted to microbiological studies. In your Annales, our laboratory research will of course occupy a central position, but the work from outside groups that you intend to publish will be a source of competitive stimulation for all of us."That first volume included 53 articles as well as critical reviews and book reviews. From that time on, the Annales appeared regularly every month, without interruption, even during the two world wars. Although the journal has undergone many changes over the past 100 years (in the title, the format, the language) reflecting the evolution in scientific publishing, it has consistently maintained the Pasteur tradition by publishing original reports on all aspects of microbiology.
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