长时间睡眠剥夺后立即恢复睡眠刺激雄性大鼠肝脏综合应激反应相关基因的转录。

IF 2.1 Q3 CLINICAL NEUROLOGY Clocks & Sleep Pub Date : 2022-11-09 DOI:10.3390/clockssleep4040048
Keisuke Fukuoka, Yusuke Murata, Tomomi Otomaru, Masayoshi Mori, Kenji Ohe, Kazunori Mine, Munechika Enjoji
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引用次数: 0

摘要

睡眠不足会导致行为障碍和疲劳。人疱疹病毒-6的再激活与真核翻译起始因子2α (eIF2α)的磷酸化有关,是用作疲劳客观生物标志物的一个候选物。磷酸化的eIF2α是细胞内应激反应系统综合应激反应(integrated stress response, ISR)的关键调控因子。然而,睡眠/睡眠不足与ISR之间的关系尚不清楚。本研究旨在探讨长时间睡眠剥夺和恢复性睡眠对大鼠肝脏isr相关基因表达的影响。八周大的雄性Sprague-Dawley大鼠使用花盆技术进行了96小时的睡眠剥夺。处死大鼠,在剥夺睡眠结束后立即或6、72 h取肝。RT-qPCR分析大鼠肝脏中isr相关基因转录本的表达水平。Atf3、Ddit3、Hmox-1和Ppp15a1r基因转录水平在睡眠剥夺结束后的恢复期早期显著升高。这些结果表明,大鼠肝脏isr的激活和失活同时发生在恢复性睡眠的早期阶段。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Recovery Sleep Immediately after Prolonged Sleep Deprivation Stimulates the Transcription of Integrated Stress Response-Related Genes in the Liver of Male Rats.

Sleep loss induces performance impairment and fatigue. The reactivation of human herpesvirus-6, which is related to the phosphorylation of eukaryotic translation initiation factor 2α (eIF2α), is one candidate for use as an objective biomarker of fatigue. Phosphorylated eIF2α is a key regulator in integrated stress response (ISR), an intracellular stress response system. However, the relation between sleep/sleep loss and ISR is unclear. The purpose of the current study was to evaluate the effect of prolonged sleep deprivation and recovery sleep on ISR-related gene expression in rat liver. Eight-week-old male Sprague-Dawley rats were subjected to a 96-hour sleep deprivation using a flowerpot technique. The rats were sacrificed, and the liver was collected immediately or 6 or 72 h after the end of the sleep deprivation. RT-qPCR was used to analyze the expression levels of ISR-related gene transcripts in the rat liver. The transcript levels of the Atf3, Ddit3, Hmox-1, and Ppp15a1r genes were markedly increased early in the recovery sleep period after the termination of sleep deprivation. These results indicate that both activation and inactivation of ISRs in the rat liver occur simultaneously in the early phase of recovery sleep.

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Clocks & Sleep
Clocks & Sleep Multiple-
CiteScore
4.40
自引率
0.00%
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审稿时长
7 weeks
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