水稻纹枯病抗性新来源及DNA标记位点鉴定

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2022-12-01 DOI:10.5423/PPJ.OA.04.2022.0054
Pachai Poonguzhali, Ashish Chauhan, Abinash Kar, Shivaji Lavale, Spurthi N Nayak, S K Prashanthi
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引用次数: 1

摘要

由坏死性土传病原菌索拉根丝核菌引起的纹枯病是对水稻生产的全球性威胁。水稻纹枯病种质资源库中缺乏稳定可靠的抗性来源,使得水稻纹枯病抗性育种工作十分困难。本研究对101个地方水稻品种进行了人工外植筛选,筛选出了Jigguvaratiga、Honasu、Jeer Sali、Jeeraga-2、BiliKagga和Medini Sannabatta 6个中等抗性的地方水稻品种,相对病害高度(RLH)在21 ~ 30%之间。采用抗性水平一致且较好(21% RLH)的长白猪吉古瓦拉蒂加(Jigguvaratiga)和抗性检查特步(Tetep) (28% RLH)建立定位群体。利用群体分离分析方法,从Jigguvaratiga × BPT5204(易感品种)的F2定位群体中鉴定出与ShB抗性相关的DNA标记。在56个亲本多态性标记中,RM5556、RM6208和RM7在群体间具有多态性。单标记分析表明,ShB与RM5556和RM6208有显著相关性,表型方差(R2)分别为28.29和20.06%。共分离分析证实了8号染色体上的RM5556和RM6208与ShB性状的强关联。这是关于RM6208标记与ShB耐药关联的首次报道。结果表明,RM6208位点存在硬脂酰ACP去饱和酶蛋白,该蛋白参与植物对病原菌的防御机制。RM5556位点驻留一个功能未知的蛋白。RM5556和RM6208标记区间的候选基因或数量性状位点可进一步利用分子育种方法培育抗ShB品种。
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New Sources of Resistance and Identification of DNA Marker Loci for Sheath Blight Disease Caused by Rhizoctonia solani Kuhn, in Rice.

Sheath blight disease caused by the necrotrophic, soilborne pathogen Rhizoctonia solani Kuhn, is the global threat to rice production. Lack of reliable stable resistance sources in rice germplasm pool for sheath blight has made resistance breeding a very difficult task. In the current study, 101 rice landraces were screened against R. solani under artificial epiphytotics and identified six moderately resistant landraces, Jigguvaratiga, Honasu, Jeer Sali, Jeeraga-2, BiliKagga, and Medini Sannabatta with relative lesion height (RLH) range of 21-30%. Landrace Jigguvaratiga with consistent and better level of resistance (21% RLH) than resistant check Tetep (RLH 28%) was used to develop mapping population. DNA markers associated with ShB resistance were identified in F2 mapping population developed from Jigguvaratiga × BPT5204 (susceptible variety) using bulk segregant analysis. Among 56 parental polymorphic markers, RM5556, RM6208, and RM7 were polymorphic between the bulks. Single marker analysis indicated the significant association of ShB with RM5556 and RM6208 with phenotypic variance (R2) of 28.29 and 20.06%, respectively. Co-segregation analysis confirmed the strong association of RM5556 and RM6208 located on chromosome 8 for ShB trait. This is the first report on association of RM6208 marker for ShB resistance. In silico analysis revealed that RM6208 loci resides the stearoyl ACP desaturases protein, which is involved in defense mechanism against plant pathogens. RM5556 loci resides a protein, with unknown function. The putative candidate genes or quantitative trait locus harbouring at the marker interval of RM5556 and RM6208 can be further used to develop ShB resistant varieties using molecular breeding approaches.

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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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