Pedram Shahrokny, Nicole Maison, Lennart Riemann, Maximilian Ehrmann, David DeLuca, Sven Schuchardt, Dominik Thiele, Markus Weckmann, Anna-Maria Dittrich, Bianca Schaub, Folke Brinkmann, Gesine Hansen, Matthias Kopp, Erika von Mutius, Klaus Rabe, Thomas Bahmer, Jens Hohlfeld, Ruth Grychtol, Olaf Holz
{"title":"全年龄哮喘队列(ALLIANCE)哮喘和喘息儿童的呼吸萘增加。","authors":"Pedram Shahrokny, Nicole Maison, Lennart Riemann, Maximilian Ehrmann, David DeLuca, Sven Schuchardt, Dominik Thiele, Markus Weckmann, Anna-Maria Dittrich, Bianca Schaub, Folke Brinkmann, Gesine Hansen, Matthias Kopp, Erika von Mutius, Klaus Rabe, Thomas Bahmer, Jens Hohlfeld, Ruth Grychtol, Olaf Holz","doi":"10.1088/1752-7163/acf23e","DOIUrl":null,"url":null,"abstract":"<p><p>Exhaled breath contains numerous volatile organic compounds (VOCs) known to be related to lung disease like asthma. Its collection is non-invasive, simple to perform and therefore an attractive method for the use even in young children. We analysed breath in children of the multicenter All Age Asthma Cohort (ALLIANCE) to evaluate if 'breathomics' have the potential to phenotype patients with asthma and wheeze, and to identify extrinsic risk factors for underlying disease mechanisms. A breath sample was collected from 142 children (asthma: 51, pre-school wheezers: 55, healthy controls: 36) and analysed using gas chromatography-mass spectrometry (GC/MS). Children were diagnosed according to Global Initiative for Asthma guidelines and comprehensively examined each year over up to seven years. Forty children repeated the breath collection after 24 or 48 months. Most breath VOCs differing between groups reflect the exposome of the children. We observed lower levels of lifestyle-related VOCs and higher levels of the environmental pollutants, especially naphthalene, in children with asthma or wheeze. Naphthalene was also higher in symptomatic patients and in wheezers with recent inhaled corticosteroid use. No relationships with lung function or TH2 inflammation were detected. Increased levels of naphthalene in asthmatics and wheezers and the relationship to disease severity could indicate a role of environmental or indoor air pollution for the development or progress of asthma. Breath VOCs might help to elucidate the role of the exposome for the development of asthma. The study was registered at ClinicalTrials.gov (NCT02496468).</p>","PeriodicalId":15306,"journal":{"name":"Journal of breath research","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2023-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Increased breath naphthalene in children with asthma and wheeze of the All Age Asthma Cohort (ALLIANCE).\",\"authors\":\"Pedram Shahrokny, Nicole Maison, Lennart Riemann, Maximilian Ehrmann, David DeLuca, Sven Schuchardt, Dominik Thiele, Markus Weckmann, Anna-Maria Dittrich, Bianca Schaub, Folke Brinkmann, Gesine Hansen, Matthias Kopp, Erika von Mutius, Klaus Rabe, Thomas Bahmer, Jens Hohlfeld, Ruth Grychtol, Olaf Holz\",\"doi\":\"10.1088/1752-7163/acf23e\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Exhaled breath contains numerous volatile organic compounds (VOCs) known to be related to lung disease like asthma. 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Naphthalene was also higher in symptomatic patients and in wheezers with recent inhaled corticosteroid use. No relationships with lung function or TH2 inflammation were detected. Increased levels of naphthalene in asthmatics and wheezers and the relationship to disease severity could indicate a role of environmental or indoor air pollution for the development or progress of asthma. Breath VOCs might help to elucidate the role of the exposome for the development of asthma. 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Increased breath naphthalene in children with asthma and wheeze of the All Age Asthma Cohort (ALLIANCE).
Exhaled breath contains numerous volatile organic compounds (VOCs) known to be related to lung disease like asthma. Its collection is non-invasive, simple to perform and therefore an attractive method for the use even in young children. We analysed breath in children of the multicenter All Age Asthma Cohort (ALLIANCE) to evaluate if 'breathomics' have the potential to phenotype patients with asthma and wheeze, and to identify extrinsic risk factors for underlying disease mechanisms. A breath sample was collected from 142 children (asthma: 51, pre-school wheezers: 55, healthy controls: 36) and analysed using gas chromatography-mass spectrometry (GC/MS). Children were diagnosed according to Global Initiative for Asthma guidelines and comprehensively examined each year over up to seven years. Forty children repeated the breath collection after 24 or 48 months. Most breath VOCs differing between groups reflect the exposome of the children. We observed lower levels of lifestyle-related VOCs and higher levels of the environmental pollutants, especially naphthalene, in children with asthma or wheeze. Naphthalene was also higher in symptomatic patients and in wheezers with recent inhaled corticosteroid use. No relationships with lung function or TH2 inflammation were detected. Increased levels of naphthalene in asthmatics and wheezers and the relationship to disease severity could indicate a role of environmental or indoor air pollution for the development or progress of asthma. Breath VOCs might help to elucidate the role of the exposome for the development of asthma. The study was registered at ClinicalTrials.gov (NCT02496468).
期刊介绍:
Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics.
Typical areas of interest include:
Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research.
Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments.
Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway.
Cellular and molecular level in vitro studies.
Clinical, pharmacological and forensic applications.
Mathematical, statistical and graphical data interpretation.