苍白芽孢杆菌PI8基因组序列推测细菌素基因簇的功能分析。

IF 0.8 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal of General and Applied Microbiology Pub Date : 2022-09-15 DOI:10.2323/jgam.2021.11.003
Kyosuke Kita, Sanako Yoshida, Shu Ishikawa, Ken-Ichi Yoshida
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引用次数: 0

摘要

细菌素是一大类由多种细菌核糖体合成的多肽。一种嗜热的革兰氏阳性细菌——苍白球芽孢杆菌PI8的基因组被发现拥有一个由五个基因组成的操纵子,可能与一种假定的细菌素的产生有关,这种细菌素被命名为pcnABCDE,用于生产“苍白球素”。本研究对pcn操纵子的功能进行了实验研究。从质粒中外源表达整个pcn操纵子对大肠杆菌有毒性,但对枯草芽孢杆菌无毒性。然而,当整个pcn操纵子组成表达时,即使枯草芽孢杆菌的生长也受到损害,至少pcnA可能是pallidocyclicin的前体。此外,一株从质粒中表达整个pcn操纵子的枯草芽孢杆菌在高温下对另一种嗜热物种kaustophilus Geobacillus显示出毒性,而另一株从pcn操纵子中单独缺乏pcnE的菌株则失去了毒性,这表明pcnE可能在枯草芽孢杆菌中产生时参与了苍白霉素的生物合成。
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Functional analysis of a gene cluster for putative bacteriocin deduced from the genome sequence of Aeribacillus pallidus PI8.

Bacteriocins are a large family of peptides synthesized ribosomally by a variety of bacterial species. The genome of one of the thermophilic Gram-positive bacteria, Aeribacillus pallidus PI8, was found to possess an operon comprising five genes possibly involved in the production of a putative bacteriocin that was named pcnABCDE for the production of "pallidocyclicin." This study investigated the function of the pcn operon experimentally. The heterologous expression of the entire pcn operon from the plasmid was toxic to Escherichia coli but not to Bacillus subtilis. However, when the entire pcn operon was expressed constitutively, even the growth of B. subtilis was impaired, and at least pcnA was implied to serve as the precursor of pallidocyclicin. In addition, a strain of B. subtilis expressing the entire pcn operon from the plasmid showed toxicity to another thermophilic species, Geobacillus kaustophilus, at elevated temperatures, whereas another strain lacking pcnE alone from the pcn operon lost the toxicity, suggesting that pcnE might be involved in the biosynthesis of pallidocyclicin when it is produced in B. subtilis.

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来源期刊
Journal of General and Applied Microbiology
Journal of General and Applied Microbiology 生物-生物工程与应用微生物
CiteScore
2.40
自引率
0.00%
发文量
42
审稿时长
6-12 weeks
期刊介绍: JGAM is going to publish scientific reports containing novel and significant microbiological findings, which are mainly devoted to the following categories: Antibiotics and Secondary Metabolites; Biotechnology and Metabolic Engineering; Developmental Microbiology; Environmental Microbiology and Bioremediation; Enzymology; Eukaryotic Microbiology; Evolution and Phylogenetics; Genome Integrity and Plasticity; Microalgae and Photosynthesis; Microbiology for Food; Molecular Genetics; Physiology and Cell Surface; Synthetic and Systems Microbiology.
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