暴露于10hz脉冲磁场诱导原代人牙龈成纤维细胞轻微凋亡和活性氧

IF 1.8 3区生物学 Q3 BIOLOGY Bioelectromagnetics Pub Date : 2022-12-09 DOI:10.1002/bem.22428

Zheng Huang, Cheng Ding, Xinzhao Huang, Chuan Sun, Liangjun Zhong

{"title":"暴露于10hz脉冲磁场诱导原代人牙龈成纤维细胞轻微凋亡和活性氧","authors":"Zheng Huang, Cheng Ding, Xinzhao Huang, Chuan Sun, Liangjun Zhong","doi":"10.1002/bem.22428","DOIUrl":null,"url":null,"abstract":"<p>Extremely low frequency pulsed magnetic fields (MFs) have been increasingly used as an effective method in oral therapy, but its potential impact on health has not been clarified. In this study, we investigated the impact of 10 Hz pulsed MF exposure on primary human gingival fibroblasts (HGFs) derived from eight healthy persons (four males and four females). Cells were exposed to 10 Hz pulsed MFs at 1.0 mT for 24 h. Cell apoptosis, cell cycle progression, intracellular reactive oxygen species levels, DNA damage, and cell proliferation were determined after exposure. The results showed that 10 Hz pulsed MFs exposure have slight effects on cellular apoptosis, cell cycle progression, and DNA damage in primary HGFs from some but not all samples. In addition, no significant effect was found on cell proliferation. © 2022 Bioelectromagnetics Society.</p>","PeriodicalId":8956,"journal":{"name":"Bioelectromagnetics","volume":"43 8","pages":"476-490"},"PeriodicalIF":1.8000,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exposure to 10 Hz Pulsed Magnetic Field Induced Slight Apoptosis and Reactive Oxygen Species in Primary Human Gingival Fibroblasts\",\"authors\":\"Zheng Huang, Cheng Ding, Xinzhao Huang, Chuan Sun, Liangjun Zhong\",\"doi\":\"10.1002/bem.22428\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Extremely low frequency pulsed magnetic fields (MFs) have been increasingly used as an effective method in oral therapy, but its potential impact on health has not been clarified. In this study, we investigated the impact of 10 Hz pulsed MF exposure on primary human gingival fibroblasts (HGFs) derived from eight healthy persons (four males and four females). Cells were exposed to 10 Hz pulsed MFs at 1.0 mT for 24 h. Cell apoptosis, cell cycle progression, intracellular reactive oxygen species levels, DNA damage, and cell proliferation were determined after exposure. The results showed that 10 Hz pulsed MFs exposure have slight effects on cellular apoptosis, cell cycle progression, and DNA damage in primary HGFs from some but not all samples. In addition, no significant effect was found on cell proliferation. © 2022 Bioelectromagnetics Society.</p>\",\"PeriodicalId\":8956,\"journal\":{\"name\":\"Bioelectromagnetics\",\"volume\":\"43 8\",\"pages\":\"476-490\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2022-12-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioelectromagnetics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/bem.22428\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioelectromagnetics","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bem.22428","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

极低频脉冲磁场(MFs)作为一种有效的口服治疗方法已被越来越多地使用，但其对健康的潜在影响尚未明确。在这项研究中，我们研究了10 Hz脉冲MF暴露对来自8名健康人(4男4女)的原代人牙龈成纤维细胞(HGFs)的影响。将细胞暴露于1.0 mT的10 Hz脉冲MFs中24小时。暴露后测定细胞凋亡、细胞周期进程、细胞内活性氧水平、DNA损伤和细胞增殖。结果表明，10 Hz脉冲MFs暴露对部分(但不是全部)原发HGFs的细胞凋亡、细胞周期进展和DNA损伤有轻微影响。此外，对细胞增殖无明显影响。©2022生物电磁学学会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

Exposure to 10 Hz Pulsed Magnetic Field Induced Slight Apoptosis and Reactive Oxygen Species in Primary Human Gingival Fibroblasts

Extremely low frequency pulsed magnetic fields (MFs) have been increasingly used as an effective method in oral therapy, but its potential impact on health has not been clarified. In this study, we investigated the impact of 10 Hz pulsed MF exposure on primary human gingival fibroblasts (HGFs) derived from eight healthy persons (four males and four females). Cells were exposed to 10 Hz pulsed MFs at 1.0 mT for 24 h. Cell apoptosis, cell cycle progression, intracellular reactive oxygen species levels, DNA damage, and cell proliferation were determined after exposure. The results showed that 10 Hz pulsed MFs exposure have slight effects on cellular apoptosis, cell cycle progression, and DNA damage in primary HGFs from some but not all samples. In addition, no significant effect was found on cell proliferation. © 2022 Bioelectromagnetics Society.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Bioelectromagnetics 生物-生物物理

CiteScore

4.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Bioelectromagnetics is published by Wiley-Liss, Inc., for the Bioelectromagnetics Society and is the official journal of the Bioelectromagnetics Society and the European Bioelectromagnetics Association. It is a peer-reviewed, internationally circulated scientific journal that specializes in reporting original data on biological effects and applications of electromagnetic fields that range in frequency from zero hertz (static fields) to the terahertz undulations and visible light. Both experimental and clinical data are of interest to the journal''s readers as are theoretical papers or reviews that offer novel insights into or criticism of contemporary concepts and theories of field-body interactions. The Bioelectromagnetics Society, which sponsors the journal, also welcomes experimental or clinical papers on the domains of sonic and ultrasonic radiation.