{"title":"多元醇对脂质体膜结构的影响:基于小角x射线散射数据和广义间接傅里叶变换的研究","authors":"Aika Sekine , Taku Ogura , Koji Tsuchiya , Kaoru Ohishi , Yuji Masubuchi , Masaaki Akamatsu , Kenichi Sakai , Masahiko Abe , Hideki Sakai","doi":"10.1016/j.chemphyslip.2022.105253","DOIUrl":null,"url":null,"abstract":"<div><p><span><span><span>This study aimed to evaluate the membrane structure<span> of distearoylphosphatidylcholine (DSPC) liposomes dispersed in water containing various types of polyols with low </span></span>molecular weight such as glycerin (Gly), 1,3-butandiol (BG), and </span>propylene<span> glycol (PG). To clarify the detailed membrane structure, generalized indirect Fourier transformation<span> (GIFT) analysis, which provides information about the bilayer spacing, bilayer thickness, number of lamellar layers, and membrane flexibility, was applied to small-angle X-ray scattering (SAXS) data of the present system. The GIFT results showed that the bilayer thickness of the DSPC liposomes followed the order Gly>>BG>PG. In addition, the membrane flexibility estimated by the Caille parameter was in the order Gly>>BG>PG; this result was supported by the gel-liquid crystal phase transition temperature (</span></span></span><em>T</em><sub>c</sub><span>) obtained by differential scanning calorimetry<span> (DSC). These results, together with the Raman spectra, suggest that BG and PG incorporated into the bilayers of DSPC liposomes result in the formation of an interdigitated lamellar structure.</span></span></p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Effect of polyols on membrane structures of liposomes: A study using small-angle X-ray scattering data and generalized indirect Fourier transformation\",\"authors\":\"Aika Sekine , Taku Ogura , Koji Tsuchiya , Kaoru Ohishi , Yuji Masubuchi , Masaaki Akamatsu , Kenichi Sakai , Masahiko Abe , Hideki Sakai\",\"doi\":\"10.1016/j.chemphyslip.2022.105253\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span><span>This study aimed to evaluate the membrane structure<span> of distearoylphosphatidylcholine (DSPC) liposomes dispersed in water containing various types of polyols with low </span></span>molecular weight such as glycerin (Gly), 1,3-butandiol (BG), and </span>propylene<span> glycol (PG). To clarify the detailed membrane structure, generalized indirect Fourier transformation<span> (GIFT) analysis, which provides information about the bilayer spacing, bilayer thickness, number of lamellar layers, and membrane flexibility, was applied to small-angle X-ray scattering (SAXS) data of the present system. The GIFT results showed that the bilayer thickness of the DSPC liposomes followed the order Gly>>BG>PG. In addition, the membrane flexibility estimated by the Caille parameter was in the order Gly>>BG>PG; this result was supported by the gel-liquid crystal phase transition temperature (</span></span></span><em>T</em><sub>c</sub><span>) obtained by differential scanning calorimetry<span> (DSC). These results, together with the Raman spectra, suggest that BG and PG incorporated into the bilayers of DSPC liposomes result in the formation of an interdigitated lamellar structure.</span></span></p></div>\",\"PeriodicalId\":275,\"journal\":{\"name\":\"Chemistry and Physics of Lipids\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2022-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chemistry and Physics of Lipids\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0009308422000810\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemistry and Physics of Lipids","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0009308422000810","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Effect of polyols on membrane structures of liposomes: A study using small-angle X-ray scattering data and generalized indirect Fourier transformation
This study aimed to evaluate the membrane structure of distearoylphosphatidylcholine (DSPC) liposomes dispersed in water containing various types of polyols with low molecular weight such as glycerin (Gly), 1,3-butandiol (BG), and propylene glycol (PG). To clarify the detailed membrane structure, generalized indirect Fourier transformation (GIFT) analysis, which provides information about the bilayer spacing, bilayer thickness, number of lamellar layers, and membrane flexibility, was applied to small-angle X-ray scattering (SAXS) data of the present system. The GIFT results showed that the bilayer thickness of the DSPC liposomes followed the order Gly>>BG>PG. In addition, the membrane flexibility estimated by the Caille parameter was in the order Gly>>BG>PG; this result was supported by the gel-liquid crystal phase transition temperature (Tc) obtained by differential scanning calorimetry (DSC). These results, together with the Raman spectra, suggest that BG and PG incorporated into the bilayers of DSPC liposomes result in the formation of an interdigitated lamellar structure.
期刊介绍：
Chemistry and Physics of Lipids publishes research papers and review articles on chemical and physical aspects of lipids with primary emphasis on the relationship of these properties to biological functions and to biomedical applications.
Accordingly, the journal covers: advances in synthetic and analytical lipid methodology; mass-spectrometry of lipids; chemical and physical characterisation of isolated structures; thermodynamics, phase behaviour, topology and dynamics of lipid assemblies; physicochemical studies into lipid-lipid and lipid-protein interactions in lipoproteins and in natural and model membranes; movement of lipids within, across and between membranes; intracellular lipid transfer; structure-function relationships and the nature of lipid-derived second messengers; chemical, physical and functional alterations of lipids induced by free radicals; enzymatic and non-enzymatic mechanisms of lipid peroxidation in cells, tissues, biofluids; oxidative lipidomics; and the role of lipids in the regulation of membrane-dependent biological processes.
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