肾移植后女性内皮祖细胞损伤的研究

IF 1.9 4区 医学 Q3 HEMATOLOGY Microcirculation Pub Date : 2022-12-09 DOI:10.1111/micc.12794
Nadia Meyer, Katja Richter, Lars Brodowski, Constantin von Kaisenberg, Anette Melk, Bernhard Schmidt, Florian P. Limbourg, Bianca Schröder-Heurich, Frauke von Versen-Höynck
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引用次数: 0

摘要

目的肾移植患者的长期生存率有了显著提高。然而,移植后发生心血管事件的风险更高,部分原因是免疫抑制。在血管生成和内皮损伤修复中发挥重要作用的内皮祖细胞(EPC)数量减少与心血管风险增加有关。本研究的目的是评估肾移植是否影响女性的EPCs。方法选择24名健康女性和22名女性肾移植受者。通过多色流式细胞术测定血管生成和非血管生成循环祖细胞(CPCs)的比例,并与临床参数相关。脐血来源的内皮集落形成细胞(ECFC)是内皮祖细胞的增殖亚群,用来自移植患者或健康对照的合并血清处理,并使用体外模型测试其功能完整性。结果与健康对照组相比,肾移植受者显示出血管生成和非血管生成CPCs的比例降低。差异在绝经前妇女中尤为明显。暴露于移植妇女的血清导致ECFC增殖、迁移和血管生成能力显著受损。结论EPC人群的改变可能导致器官移植后心血管风险的增加,应在治疗策略中加以考虑。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Impairment of endothelial progenitor cells in women after kidney transplantation

Objective

The long-term survival of kidney transplant patients has substantially improved. However, there is a higher risk for cardiovascular events after transplantation, partly due to immunosuppression. A diminished number of endothelial progenitor cells (EPCs), which play an important role in angiogenesis and the repair of endothelial damage, are associated with an increased cardiovascular risk. The aim of this study was to evaluate whether kidney transplantation affects EPCs in women.

Methods

Twenty-four healthy women and 22 female kidney transplant recipients were recruited. The ratio of angiogenic and non-angiogenic circulating progenitor cells (CPCs) was determined by multicolor flow cytometry and related to clinical parameters. Cord blood-derived endothelial colony-forming cells (ECFCs), a proliferative subgroup of endothelial progenitor cells, were treated with pooled sera from transplant patients or healthy controls and tested for their functional integrity using in vitro models.

Results

Kidney transplant recipients displayed a reduced ratio of angiogenic and non-angiogenic CPCs compared to healthy controls. Differences were especially pronounced in premenopausal women. Exposure to sera of transplanted women led to a significant impairment of ECFC proliferation, migration, and angiogenesis ability.

Conclusions

Alterations of EPC populations may contribute to the higher cardiovascular risks after organ transplantation and should be considered in therapeutic strategies.

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来源期刊
Microcirculation
Microcirculation 医学-外周血管病
CiteScore
5.00
自引率
4.20%
发文量
43
审稿时长
6-12 weeks
期刊介绍: The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.
期刊最新文献
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