通过抑制TLR4/NF-κB通路和NLRP3炎性体,敲低TRIM62可减轻糖尿病小鼠认知功能障碍。

IF 2 4区 医学 Q3 NUTRITION & DIETETICS Journal of Clinical Biochemistry and Nutrition Pub Date : 2023-09-01 DOI:10.3164/jcbn.22-104
Xiting Nong, Nan Li, Xiang Wang, Heng Li, Xiaoping Wu, Ming Li, Wenqing Hao, Guang Yang
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引用次数: 0

摘要

tripartite motif 62是一种E3泛素连接酶蛋白,调节细胞过程,包括分化、免疫、发育和凋亡,导致各种疾病状态,如癌症和炎症性疾病。然而,三方基序62在糖尿病引起的认知障碍过程中的作用和机制尚未见报道。因此,本研究的目的是探讨三方基序62在糖尿病诱导的认知障碍中的作用和机制。结果表明,糖尿病小鼠中三边基序62的表达上调。沉默TRIM62使糖尿病小鼠体重增加,空腹血糖降低。此外,敲低trpartite motif 62可以抑制stz诱导的炎症、细胞凋亡和氧化应激。进一步研究发现,TLR4/NF-κB通路和NLRP3炎性小体通过三方基序62参与糖尿病小鼠的调节。更重要的是,抑制三边基序62可改善小鼠的认知障碍和学习能力。综上所述,抑制TRIM62可抑制stz诱导的炎症、细胞凋亡和氧化应激,提高小鼠的认知能力。因此,三方基序62可能是治疗糖尿病引起的认知障碍的重要靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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TRIM62 knockdown by inhibiting the TLR4/NF-κB pathway and NLRP3 inflammasome attenuates cognitive impairment induced by diabetes in mice.

The tripartite motif 62 is an E3 ubiquitin ligase protein that regulates cellular processes, including differentiation, immunity, development and apoptosis, leading to various disease states, such as cancer and inflammatory diseases. However, the role and mechanism of the tripartite motif 62 in the process of diabetic-induced cognitive impairment have not been reported. Therefore, the aim of this study was to investigate the role and mechanism of the tripartite motif 62 in diabetic-induced cognitive impairment. The results showed that the expression of the tripartite motif 62 was up-regulated in diabetic mice. Silencing of TRIM62 increased body weight and decreased fasting blood glucose in diabetic mice. In addition, knockdown of the tripartite motif 62 inhibited STZ-induced inflammation, apoptosis and oxidative stress. Further studies showed that the TLR4/NF-κB pathway and NLRP3 inflammasomes were involved in the regulation of diabetic mice by the tripartite motif 62. More importantly, inhibition of the tripartite motif 62 improved cognitive impairment and learning ability in mice. In conclusion, inhibition of TRIM62 inhibits STZ-induced inflammation, cell apoptosis and oxidative stress, and improves the cognitive ability of mice. Therefore, the tripartite motif 62 may be an important target for the treatment of diabetes-induced cognitive impairment.

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来源期刊
CiteScore
4.30
自引率
8.30%
发文量
57
审稿时长
6-12 weeks
期刊介绍: Journal of Clinical Biochemistry and Nutrition (JCBN) is an international, interdisciplinary publication encompassing chemical, biochemical, physiological, pathological, toxicological and medical approaches to research on lipid peroxidation, free radicals, oxidative stress and nutrition. The Journal welcomes original contributions dealing with all aspects of clinical biochemistry and clinical nutrition including both in vitro and in vivo studies.
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