LncRNA FAM225B通过调节转录因子DDX17调节PDIA4介导的卵巢癌症细胞侵袭和迁移。

IF 1.9 4区医学 Q3 OBSTETRICS & GYNECOLOGY Breast Journal Pub Date : 2023-09-07 eCollection Date: 2023-01-01 DOI:10.1155/2023/3970444

Chanjiao Yao, Lingjuan Zeng, Qin Liu, Xiaoxin Qiu, Chunyan Chen

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引用次数: 0

摘要

目的：探讨lncRNA FAM225B和PDIA4在癌症中的作用及其机制。方法：采用RT-qPCR和Western印迹法检测卵巢癌症患者血清和细胞系中lncRNAs FAM225B、DDX17和PDIA4的表达水平。用lncRNA FAM225B-和PDIA4相关载体转染细胞，以使用功能实验确定恶性表型。使用RNA下拉和RIP分析验证lncRNA FAM225B和DDX17的相互结合。结果：lncRNAs FAM225B和PDIA4在癌症患者血清和细胞系中的表达降低。lncRNA FAM225B或PDIA4的恢复降低了细胞增殖、迁移和侵袭能力，并提高了细胞凋亡率，而lncRNA FAM225B或PDIA4的抑制表现出相反的趋势。RNA下拉和RIP分析揭示了lncRNA FAM225B和DDX17之间的直接相互作用。ChIP分析揭示了DDX17和PDIA4启动子之间的关系。LncRNA FAM225B和DDX17正调控PDIA4的表达。PDIA4表达的下调抵消了lncRNA FAM225B在卵巢癌症细胞中过表达的抑制作用。结论：本研究支持lncRNA FAM225B在卵巢癌症中通过直接结合DDX17上调PDIA4，抑制卵巢癌症细胞的活性。

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LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17.

Objective: This study aimed to explore the roles and mechanisms of lncRNA FAM225B and PDIA4 in ovarian cancer.

Methods: RT-qPCR and Western blot assays were performed to detect the expression levels of the lncRNAs FAM225B, DDX17, and PDIA4 in the serum of patients with ovarian cancer and cell lines. Cells were transfected with lncRNA FAM225B- and PDIA4-related vectors to determine the malignant phenotypes using functional experiments. The mutual binding of lncRNA FAM225B and DDX17 was verified using RNA pull-down and RIP assays.

Results: The expression of lncRNAs FAM225B and PDIA4 was decreased in the serum of patients with ovarian cancer and cell lines. Restoration of lncRNA FAM225B or PDIA4 reduced cell proliferation, migration, and invasion abilities and elevated the apoptosis rate, whereas suppression of lncRNA FAM225B or PDIA4 exhibited an inverse trend. RNA pull-down and RIP assays revealed a direct interaction between lncRNA FAM225B and DDX17. ChIP assay revealed a relationship between DDX17 and the PDIA4 promoter. LncRNA FAM225B and DDX17 positively regulate PDIA4 expression. Downregulation of PDIA4 expression counteracts the suppressive effect of lncRNA FAM225B overexpression in ovarian cancer cells.

Conclusion: This research study supports the fact that lncRNA FAM225B in ovarian cancer can upregulate PDIA4 by directly binding to DDX17, inhibiting the activities of ovarian cancer cells.

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来源期刊

Breast Journal 医学-妇产科学

CiteScore

4.00

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： The Breast Journal is the first comprehensive, multidisciplinary source devoted exclusively to all facets of research, diagnosis, and treatment of breast disease. The Breast Journal encompasses the latest news and technologies from the many medical specialties concerned with breast disease care in order to address the disease within the context of an integrated breast health care. This editorial philosophy recognizes the special social, sexual, and psychological considerations that distinguish cancer, and breast cancer in particular, from other serious diseases. Topics specifically within the scope of The Breast Journal include: Risk Factors Prevention Early Detection Diagnosis and Therapy Psychological Issues Quality of Life Biology of Breast Cancer.