昆虫PIF/Harbinger转座因子蛋白的反复共驯化。

IF 4.7 2区 生物学 Q1 GENETICS & HEREDITY Mobile DNA Pub Date : 2022-11-30 DOI:10.1186/s13100-022-00282-2
Dragomira N Markova, Fatema B Ruma, Claudio Casola, Ayda Mirsalehi, Esther Betrán
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引用次数: 1

摘要

背景:转座因子(te)是一种自私的DNA序列,能够以牺牲宿主细胞为代价移动和扩增。尽管如此,越来越多的研究表明,TE蛋白是通过分子驯化产生新的宿主蛋白的重要贡献者。我们之前在果蝇中描述了来自TEs的PIF/Harbinger DNA家族的7个转座酶衍生的驯化基因和共同驯化。所有已知的植物和动物中的PIF te都是通过两个基因的存在将自己与其他DNA转座子区分开来的。我们假设,由于转座酶(基因1)被MADF蛋白(基因2)易位到细胞核中,因此来自同一TE的两个基因应该经常共同驯化。为了支持这一新基因起源模型,我们研究了现有的昆虫物种基因组,以寻找PIF TE驯化事件的额外证据,并探索了来自同一TE插入的MADF蛋白的共同驯化。结果:在广泛的昆虫物种基因组探索了PIF转座的撞击并分析了它们的背景和进化之后,我们提出了至少六个独立的PIF转座元件蛋白在昆虫中的驯化事件的证据:在按蚊(双翅目)中发生了两次转座酶和MADF蛋白的共驯化事件,在蝴蝶和飞蛾(鳞翅目)中发生了一次单转座酶驯化事件和一次共驯化事件,在蟑螂(斑总目)中发生了两次单转座酶驯化事件。许多这些蛋白的预测核定位信号和在某些情况下的双双转录支持共驯化转座酶和MADF蛋白的功能关联。结论:我们的研究结果增加了我们之前在果蝇基因组中描述的共同驯化,并支持通过PIF转座酶的驯化产生的新基因在昆虫中经常伴随着同源MADF蛋白的共同驯化,可能具有调节功能。我们提出了一个详细的模型,预测PIF - TE蛋白的共驯化应该经常发生在相同的PIF - TE插入中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Recurrent co-domestication of PIF/Harbinger transposable element proteins in insects.

Background: Transposable elements (TEs) are selfish DNA sequences capable of moving and amplifying at the expense of host cells. Despite this, an increasing number of studies have revealed that TE proteins are important contributors to the emergence of novel host proteins through molecular domestication. We previously described seven transposase-derived domesticated genes from the PIF/Harbinger DNA family of TEs in Drosophila and a co-domestication. All PIF TEs known in plants and animals distinguish themselves from other DNA transposons by the presence of two genes. We hypothesize that there should often be co-domestications of the two genes from the same TE because the transposase (gene 1) has been described to be translocated to the nucleus by the MADF protein (gene 2). To provide support for this model of new gene origination, we investigated available insect species genomes for additional evidence of PIF TE domestication events and explored the co-domestication of the MADF protein from the same TE insertion.

Results: After the extensive insect species genomes exploration of hits to PIF transposases and analyses of their context and evolution, we present evidence of at least six independent PIF transposable elements proteins domestication events in insects: two co-domestications of both transposase and MADF proteins in Anopheles (Diptera), one transposase-only domestication event and one co-domestication in butterflies and moths (Lepidoptera), and two transposases-only domestication events in cockroaches (Blattodea). The predicted nuclear localization signals for many of those proteins and dicistronic transcription in some instances support the functional associations of co-domesticated transposase and MADF proteins.

Conclusions: Our results add to a co-domestication that we previously described in fruit fly genomes and support that new gene origination through domestication of a PIF transposase is frequently accompanied by the co-domestication of a cognate MADF protein in insects, potentially for regulatory functions. We propose a detailed model that predicts that PIF TE protein co-domestication should often occur from the same PIF TE insertion.

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来源期刊
Mobile DNA
Mobile DNA GENETICS & HEREDITY-
CiteScore
8.20
自引率
6.10%
发文量
26
审稿时长
11 weeks
期刊介绍: Mobile DNA is an online, peer-reviewed, open access journal that publishes articles providing novel insights into DNA rearrangements in all organisms, ranging from transposition and other types of recombination mechanisms to patterns and processes of mobile element and host genome evolution. In addition, the journal will consider articles on the utility of mobile genetic elements in biotechnological methods and protocols.
期刊最新文献
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