Nnaemeka C. Iriemenam , Fehintola A. Ige , Stacie M. Greby , Olumide O. Okunoye , Mabel Uwandu , Maureen Aniedobe , Stephnie O. Nwaiwu , Nwando Mba , Mary Okoli , Nwachukwu E. William , Akipu Ehoche , Augustine Mpamugo , Andrew Mitchell , Kristen A. Stafford , Andrew N. Thomas , Temitope Olaleye , Oluwaseun O. Akinmulero , Ndidi P. Agala , Ado G. Abubakar , Ajile Owens , Rosemary Audu
{"title":"尼日利亚一种单抗原和三种多抗原严重急性呼吸系统综合征冠状病毒2型IgG检测的比较","authors":"Nnaemeka C. Iriemenam , Fehintola A. Ige , Stacie M. Greby , Olumide O. Okunoye , Mabel Uwandu , Maureen Aniedobe , Stephnie O. Nwaiwu , Nwando Mba , Mary Okoli , Nwachukwu E. William , Akipu Ehoche , Augustine Mpamugo , Andrew Mitchell , Kristen A. Stafford , Andrew N. Thomas , Temitope Olaleye , Oluwaseun O. Akinmulero , Ndidi P. Agala , Ado G. Abubakar , Ajile Owens , Rosemary Audu","doi":"10.1016/j.jcvp.2023.100139","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>Determining an accurate estimate of SARS-CoV-2 seroprevalence has been challenging in African countries where malaria and other pathogens are endemic. We compared the performance of one single-antigen assay and three multi-antigen SARS-CoV-2 IgG assays in a Nigerian population endemic for malaria.</p></div><div><h3>Methods</h3><p>De-identified plasma specimens from SARS-CoV-2 RT-PCR positive, dried blood spot (DBS) SARS-CoV-2 RT-PCR positive, and pre-pandemic negatives were used to evaluate the performance of the four SARS-CoV-2 assays (Tetracore, SARS2MBA, RightSign, xMAP).</p></div><div><h3>Results</h3><p>Results showed higher sensitivity with the multi-antigen (81% (Tetracore), 96% (SARS2MBA), 85% (xMAP)) versus the single-antigen (RightSign (64%)) SARS-CoV-2 assay. The overall specificities were 98% (Tetracore), 100% (SARS2MBA and RightSign), and 99% (xMAP). When stratified based on <15 days to ≥15 days post-RT-PCR confirmation, the sensitivities increased from 75% to 88.2% for Tetracore; from 93% to 100% for the SARS2MBA; from 58% to 73% for RightSign; and from 83% to 88% for xMAP. With DBS, there was no positive increase after 15-28 days for the three assays (Tetracore, SARS2MBA, and xMAP).</p></div><div><h3>Conclusion</h3><p>Multi-antigen assays performed well in Nigeria, even with samples with known malaria reactivity, and might provide more accurate measures of COVID-19 seroprevalence and vaccine efficacy.</p></div>","PeriodicalId":73673,"journal":{"name":"Journal of clinical virology plus","volume":"3 1","pages":"Article 100139"},"PeriodicalIF":1.6000,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837382/pdf/","citationCount":"0","resultStr":"{\"title\":\"Comparison of one single-antigen assay and three multi-antigen SARS-CoV-2 IgG assays in Nigeria\",\"authors\":\"Nnaemeka C. Iriemenam , Fehintola A. Ige , Stacie M. Greby , Olumide O. Okunoye , Mabel Uwandu , Maureen Aniedobe , Stephnie O. Nwaiwu , Nwando Mba , Mary Okoli , Nwachukwu E. William , Akipu Ehoche , Augustine Mpamugo , Andrew Mitchell , Kristen A. Stafford , Andrew N. Thomas , Temitope Olaleye , Oluwaseun O. Akinmulero , Ndidi P. Agala , Ado G. Abubakar , Ajile Owens , Rosemary Audu\",\"doi\":\"10.1016/j.jcvp.2023.100139\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>Determining an accurate estimate of SARS-CoV-2 seroprevalence has been challenging in African countries where malaria and other pathogens are endemic. We compared the performance of one single-antigen assay and three multi-antigen SARS-CoV-2 IgG assays in a Nigerian population endemic for malaria.</p></div><div><h3>Methods</h3><p>De-identified plasma specimens from SARS-CoV-2 RT-PCR positive, dried blood spot (DBS) SARS-CoV-2 RT-PCR positive, and pre-pandemic negatives were used to evaluate the performance of the four SARS-CoV-2 assays (Tetracore, SARS2MBA, RightSign, xMAP).</p></div><div><h3>Results</h3><p>Results showed higher sensitivity with the multi-antigen (81% (Tetracore), 96% (SARS2MBA), 85% (xMAP)) versus the single-antigen (RightSign (64%)) SARS-CoV-2 assay. The overall specificities were 98% (Tetracore), 100% (SARS2MBA and RightSign), and 99% (xMAP). When stratified based on <15 days to ≥15 days post-RT-PCR confirmation, the sensitivities increased from 75% to 88.2% for Tetracore; from 93% to 100% for the SARS2MBA; from 58% to 73% for RightSign; and from 83% to 88% for xMAP. With DBS, there was no positive increase after 15-28 days for the three assays (Tetracore, SARS2MBA, and xMAP).</p></div><div><h3>Conclusion</h3><p>Multi-antigen assays performed well in Nigeria, even with samples with known malaria reactivity, and might provide more accurate measures of COVID-19 seroprevalence and vaccine efficacy.</p></div>\",\"PeriodicalId\":73673,\"journal\":{\"name\":\"Journal of clinical virology plus\",\"volume\":\"3 1\",\"pages\":\"Article 100139\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2023-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9837382/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of clinical virology plus\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2667038023000066\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of clinical virology plus","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667038023000066","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Comparison of one single-antigen assay and three multi-antigen SARS-CoV-2 IgG assays in Nigeria
Objectives
Determining an accurate estimate of SARS-CoV-2 seroprevalence has been challenging in African countries where malaria and other pathogens are endemic. We compared the performance of one single-antigen assay and three multi-antigen SARS-CoV-2 IgG assays in a Nigerian population endemic for malaria.
Methods
De-identified plasma specimens from SARS-CoV-2 RT-PCR positive, dried blood spot (DBS) SARS-CoV-2 RT-PCR positive, and pre-pandemic negatives were used to evaluate the performance of the four SARS-CoV-2 assays (Tetracore, SARS2MBA, RightSign, xMAP).
Results
Results showed higher sensitivity with the multi-antigen (81% (Tetracore), 96% (SARS2MBA), 85% (xMAP)) versus the single-antigen (RightSign (64%)) SARS-CoV-2 assay. The overall specificities were 98% (Tetracore), 100% (SARS2MBA and RightSign), and 99% (xMAP). When stratified based on <15 days to ≥15 days post-RT-PCR confirmation, the sensitivities increased from 75% to 88.2% for Tetracore; from 93% to 100% for the SARS2MBA; from 58% to 73% for RightSign; and from 83% to 88% for xMAP. With DBS, there was no positive increase after 15-28 days for the three assays (Tetracore, SARS2MBA, and xMAP).
Conclusion
Multi-antigen assays performed well in Nigeria, even with samples with known malaria reactivity, and might provide more accurate measures of COVID-19 seroprevalence and vaccine efficacy.
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