{"title":"水飞蓟提取物负载壳聚糖纳米颗粒的合成特征和神经保护作用","authors":"Hatice Feyzan Ay, Serap Yesilkir-Baydar, Rabia Cakir-Koc","doi":"10.1080/02652048.2023.2167012","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong><i>Silybum marianum</i> extract (SME) possesses neuroprotective potency through its high antioxidant content. We attempted to increase the effectiveness of SME by encapsulating them in chitosan. Neuroprotective potency of SME and SME-loaded chitosan nanoparticles (SME-CNPs) were shown in SH-SY5Y cell line against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress.</p><p><strong>Methods: </strong>We produced CNPs and SME-CNPs by ionic gelation method and properly determined their physical characteristics. Encapsulation efficiency, loading capacity, and <i>in vitro</i> release tests were performed for SME-CNPs. The neurotoxicity and neuroprotective efficiency in SH-SY5Y cell line against H<sub>2</sub>O<sub>2</sub> was also investigated.</p><p><strong>Results: </strong>The size of SME-CNPs was 168.2 ± 11.12 nm with zeta potential 10.6 ± 1.0 mV. The encapsulation efficiency and loading capacity were successfully achieved at 96.6% and 1.89% respectively. SME and SME-CNPs improved cell viability higher than 80%, and SME-CNPs exhibited significant neuroprotective effects against H<sub>2</sub>O<sub>2</sub> damage.</p><p><strong>Conclusions: </strong>It was concluded that SME and SME-CNPs highly prevent damage caused by H<sub>2</sub>O<sub>2</sub> and reduce cell damage <i>in vitro</i> by their neuroprotective effects.</p>","PeriodicalId":16391,"journal":{"name":"Journal of microencapsulation","volume":"40 1","pages":"29-36"},"PeriodicalIF":3.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Synthesis characterisation and neuroprotectivity of <i>Silybum marianum</i> extract loaded chitosan nanoparticles.\",\"authors\":\"Hatice Feyzan Ay, Serap Yesilkir-Baydar, Rabia Cakir-Koc\",\"doi\":\"10.1080/02652048.2023.2167012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong><i>Silybum marianum</i> extract (SME) possesses neuroprotective potency through its high antioxidant content. We attempted to increase the effectiveness of SME by encapsulating them in chitosan. Neuroprotective potency of SME and SME-loaded chitosan nanoparticles (SME-CNPs) were shown in SH-SY5Y cell line against H<sub>2</sub>O<sub>2</sub>-induced oxidative stress.</p><p><strong>Methods: </strong>We produced CNPs and SME-CNPs by ionic gelation method and properly determined their physical characteristics. Encapsulation efficiency, loading capacity, and <i>in vitro</i> release tests were performed for SME-CNPs. The neurotoxicity and neuroprotective efficiency in SH-SY5Y cell line against H<sub>2</sub>O<sub>2</sub> was also investigated.</p><p><strong>Results: </strong>The size of SME-CNPs was 168.2 ± 11.12 nm with zeta potential 10.6 ± 1.0 mV. The encapsulation efficiency and loading capacity were successfully achieved at 96.6% and 1.89% respectively. SME and SME-CNPs improved cell viability higher than 80%, and SME-CNPs exhibited significant neuroprotective effects against H<sub>2</sub>O<sub>2</sub> damage.</p><p><strong>Conclusions: </strong>It was concluded that SME and SME-CNPs highly prevent damage caused by H<sub>2</sub>O<sub>2</sub> and reduce cell damage <i>in vitro</i> by their neuroprotective effects.</p>\",\"PeriodicalId\":16391,\"journal\":{\"name\":\"Journal of microencapsulation\",\"volume\":\"40 1\",\"pages\":\"29-36\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of microencapsulation\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/02652048.2023.2167012\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/1/25 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, APPLIED\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microencapsulation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/02652048.2023.2167012","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/25 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
Synthesis characterisation and neuroprotectivity of Silybum marianum extract loaded chitosan nanoparticles.
Aim: Silybum marianum extract (SME) possesses neuroprotective potency through its high antioxidant content. We attempted to increase the effectiveness of SME by encapsulating them in chitosan. Neuroprotective potency of SME and SME-loaded chitosan nanoparticles (SME-CNPs) were shown in SH-SY5Y cell line against H2O2-induced oxidative stress.
Methods: We produced CNPs and SME-CNPs by ionic gelation method and properly determined their physical characteristics. Encapsulation efficiency, loading capacity, and in vitro release tests were performed for SME-CNPs. The neurotoxicity and neuroprotective efficiency in SH-SY5Y cell line against H2O2 was also investigated.
Results: The size of SME-CNPs was 168.2 ± 11.12 nm with zeta potential 10.6 ± 1.0 mV. The encapsulation efficiency and loading capacity were successfully achieved at 96.6% and 1.89% respectively. SME and SME-CNPs improved cell viability higher than 80%, and SME-CNPs exhibited significant neuroprotective effects against H2O2 damage.
Conclusions: It was concluded that SME and SME-CNPs highly prevent damage caused by H2O2 and reduce cell damage in vitro by their neuroprotective effects.
期刊介绍：
The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation.
The journal covers:
Chemistry of encapsulation materials
Physics of release through the capsule wall and/or desorption from carrier
Techniques of preparation, content and storage
Many uses to which microcapsules are put.
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