微生物天冬氨酸蛋白酶的分子改造和生物技术应用。

IF 8.1 2区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Critical Reviews in Biotechnology Pub Date : 2024-05-01 Epub Date: 2023-02-26 DOI:10.1080/07388551.2023.2171850
Richard Ansah Herman, Ellen Ayepa, Wen-Xin Zhang, Zong-Nan Li, Xuan Zhu, Michael Ackah, Shuang-Shuang Yuan, Shuai You, Jun Wang
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引用次数: 0

摘要

由于微生物天冬氨酸蛋白酶具有高催化功能和对底物的高度选择性,因此越来越多的企业倾向于在工业中使用这种酶。然而,这些特性可归因于其结构的分子改变和其他各种特征。分子工具、功能基因组学和基因组编辑技术与其他生物技术方法相结合,帮助提高了具有工业重要性的微生物蛋白酶的潜力,解决了它们的一些主要局限性,如:催化效率低、转化率低、热稳定性低和酶产量少。然而,蛋白酶全域内的原生折叠依赖于周围的结构,这就对它们在底物转化中的功能提出了挑战,这主要是由于它们在复杂系统中的相互影响。因此,操纵它们的结构和控制它们的表达系统有可能产生具有高选择性和催化功能的酶。微生物天冬氨酸蛋白酶产生的蛋白质在工业上能够调节某些有害的独特工业过程,并具有生态友好的优点,意义深远。本综述介绍了当前微生物蛋白酶生物技术的最新趋势和差距,探讨了广泛应用于微生物天冬氨酸蛋白酶工程表达平台的相关重组策略和分子技术,以及其潜在的工业和生物技术应用。
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Molecular modification and biotechnological applications of microbial aspartic proteases.

The growing preference for incorporating microbial aspartic proteases in industries is due to their high catalytic function and high degree of substrate selectivity. These properties, however, are attributable to molecular alterations in their structure and a variety of other characteristics. Molecular tools, functional genomics, and genome editing technologies coupled with other biotechnological approaches have aided in improving the potential of industrially important microbial proteases by addressing some of their major limitations, such as: low catalytic efficiency, low conversion rates, low thermostability, and less enzyme yield. However, the native folding within their full domain is dependent on a surrounding structure which challenges their functionality in substrate conversion, mainly due to their mutual interactions in the context of complex systems. Hence, manipulating their structure and controlling their expression systems could potentially produce enzymes with high selectivity and catalytic functions. The proteins produced by microbial aspartic proteases are industrially capable and far-reaching in regulating certain harmful distinctive industrial processes and the benefits of being eco-friendly. This review provides: an update on current trends and gaps in microbial protease biotechnology, exploring the relevant recombinant strategies and molecular technologies widely used in expression platforms for engineering microbial aspartic proteases, as well as their potential industrial and biotechnological applications.

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来源期刊
Critical Reviews in Biotechnology
Critical Reviews in Biotechnology 工程技术-生物工程与应用微生物
CiteScore
20.80
自引率
1.10%
发文量
71
审稿时长
4.8 months
期刊介绍: Biotechnological techniques, from fermentation to genetic manipulation, have become increasingly relevant to the food and beverage, fuel production, chemical and pharmaceutical, and waste management industries. Consequently, academic as well as industrial institutions need to keep abreast of the concepts, data, and methodologies evolved by continuing research. This journal provides a forum of critical evaluation of recent and current publications and, periodically, for state-of-the-art reports from various geographic areas around the world. Contributing authors are recognized experts in their fields, and each article is reviewed by an objective expert to ensure accuracy and objectivity of the presentation.
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