线虫的染色质结构调控。

IF 4.2 2区 生物学 Q1 GENETICS & HEREDITY Epigenetics & Chromatin Pub Date : 2022-11-29 DOI:10.1186/s13072-022-00472-5
John L Carter, Colton E Kempton, Emily D S Hales, Steven M Johnson
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引用次数: 0

摘要

背景:核小体介导的染色质压缩直接影响反式激活因子和抑制因子对DNA靶标的可及性,并作为遗传表达的主要调节因子。了解染色质的性质和动力学是阐明表观遗传调控基因表达的机制和因素的基础。先前的研究表明,有三种典型序列强烈地调节核小体的定位和染色质的可及性:假定的核小体定位元件、假定的核小体排斥序列和A/T的同聚序列。据推测,这些元件可用于线虫的染色质重塑。在这里,我们展示了这些元素在体内的效用,以及新发现的排斥序列PRS-322的极端功效。结果:在这项工作中,我们表明有可能仅使用规范和假定的定位序列来操纵秀丽隐杆线虫的核小体定位。我们不仅测试了先前描述的序列,如Widom 601,而且还测试了其他核小体定位序列:Trifonov序列,推定的排斥序列-322 (PRS-322),以及A和T核苷酸的各种同聚序列。结论:利用这些假定的核小体定位序列中的每一种,我们证明了它们能够改变秀丽隐杆线虫的核小体特征,这可以通过改变体内核小体的占用和定位来证明。此外,我们还展示了PRS-322对核小体排斥和染色质重塑的影响。
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Manipulating chromatin architecture in C. elegans.

Background: Nucleosome-mediated chromatin compaction has a direct effect on the accessibility of trans-acting activators and repressors to DNA targets and serves as a primary regulatory agent of genetic expression. Understanding the nature and dynamics of chromatin is fundamental to elucidating the mechanisms and factors that epigenetically regulate gene expression. Previous work has shown that there are three types of canonical sequences that strongly regulate nucleosome positioning and thus chromatin accessibility: putative nucleosome-positioning elements, putative nucleosome-repelling sequences, and homopolymeric runs of A/T. It is postulated that these elements can be used to remodel chromatin in C. elegans. Here we show the utility of such elements in vivo, and the extreme efficacy of a newly discovered repelling sequence, PRS-322.

Results: In this work, we show that it is possible to manipulate nucleosome positioning in C. elegans solely using canonical and putative positioning sequences. We have not only tested previously described sequences such as the Widom 601, but also have tested additional nucleosome-positioning sequences: the Trifonov sequence, putative repelling sequence-322 (PRS-322), and various homopolymeric runs of A and T nucleotides.

Conclusions: Using each of these types of putative nucleosome-positioning sequences, we demonstrate their ability to alter the nucleosome profile in C. elegans as evidenced by altered nucleosome occupancy and positioning in vivo. Additionally, we show the effect that PRS-322 has on nucleosome-repelling and chromatin remodeling.

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来源期刊
Epigenetics & Chromatin
Epigenetics & Chromatin GENETICS & HEREDITY-
CiteScore
7.00
自引率
0.00%
发文量
35
审稿时长
1 months
期刊介绍: Epigenetics & Chromatin is a peer-reviewed, open access, online journal that publishes research, and reviews, providing novel insights into epigenetic inheritance and chromatin-based interactions. The journal aims to understand how gene and chromosomal elements are regulated and their activities maintained during processes such as cell division, differentiation and environmental alteration.
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