禽流感病毒在鸵鸟胚中的繁殖。

IF 1.5 3区 农林科学 Q2 VETERINARY SCIENCES Onderstepoort Journal of Veterinary Research Pub Date : 2022-12-08 DOI:10.4102/ojvr.v89i1.2011
Agnes T Laleye, Modupeore Adeyemi, Celia Abolnik
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引用次数: 0

摘要

甲型流感病毒(iav)通常通过使用9至11日龄的有胚鸡蛋(ECEs)和14日龄的有胚鸡蛋(ECEs)连续传代来分离和培养,以进行病毒突变研究。实时逆转录聚合酶链反应试验(rt - pcr)通常用于IAV诊断,但病毒分离在其高灵敏度方面仍然是无价的,为进一步的研究提供了活的分离物,并能够区分活的和不活的病毒。利用ECEs从RT-PCR阳性标本中分离鸵鸟来源的iav的努力往往不成功,这增加了物种瓶颈的可能性,即适应鸵鸟的iav可能不容易在ECEs中感染和复制,但鸵鸟胚胎支持流感病毒复制的能力先前尚未得到证实。本研究描述了一种优化的H5和H7亚型IAV在28日龄(相当于14日龄)的胚胎鸵鸟蛋(EOEs)中分离和繁殖的方法。在长途运输前对卵进行12 ~ 14天的预孵育,可最大限度地提高卵的存活率。将这种方法应用于鸵鸟适应病毒分离研究和鸟蛋研究,将有助于更好地了解鸵鸟中病毒与宿主的相互作用和潜在人畜共患疾病的出现。
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Propagation of avian influenza virus in embryonated ostrich eggs.

Influenza A viruses (IAVs) are typically isolated and cultured by successive passages using 9- to 11-day-old embryonated chicken eggs (ECEs) and in 14-day old ECEs for virus mutational studies. Real-time reverse transcription-polymerase chain reaction tests (RT-PCRs) are commonly used for IAV diagnosis, but virus isolation remains invaluable in terms of its high sensitivity, providing viable isolates for further studies and the ability to distinguish between viable and nonviable virus. Efforts at isolating ostrich-origin IAVs from RT-PCR positive specimens using ECEs have often been unsuccessful, raising the possibility of a species bottleneck, whereby ostrich-adapted IAVs may not readily infect and replicate in ECEs, yet the capacity of an ostrich embryo to support the replication of influenza viruses has not been previously demonstrated. This study describes an optimised method for H5 and H7 subtype IAV isolation and propagation in 28-day old embryonated ostrich eggs (EOEs), the biological equivalent of 14-day old ECEs. The viability of EOEs transported from breeding sites could be maximised by pre-incubating the eggs for 12 to 14 days prior to long-distance transportation. This method applied to studies for ostrich-adapted virus isolation and in ovo studies will enable better understanding of the virus-host interaction in ostriches and the emergence of potentially zoonotic diseases.

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来源期刊
CiteScore
4.30
自引率
0.00%
发文量
13
审稿时长
16 weeks
期刊介绍: The Onderstepoort Journal of Veterinary Research, is the official publication of the Onderstepoort Veterinary Institute. While it considers submissions from any geographic region, its focus is on Africa and the infectious and parasitic diseases and disease vectors that affect livestock and wildlife on the continent.
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