野生型单纯疱疹病毒诱导的细胞-细胞融合系统的建立揭示了HSV-1包膜糖蛋白B n -糖基化在细胞-细胞融合中的作用

IF 1.9 4区医学 Q4 IMMUNOLOGY Microbiology and Immunology Pub Date : 2023-01-06 DOI:10.1111/1348-0421.13050

Ayano Fukui, Yuhei Maruzuru, Kosuke Takeshima, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi

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引用次数: 2

摘要

野生型单纯疱疹病毒(HSV)株在细胞培养中很少介导细胞-细胞融合，很少诱导大的多核细胞。在这项研究中，我们建立了一个系统来量化野生型HSV菌株诱导的罕见细胞-细胞融合。建立的系统明确了HSV-1包膜糖蛋白B及其在141位天冬酰胺上的n -糖基化是细胞-细胞有效融合所必需的。本研究提供了野生型HSV-1诱导的细胞-细胞融合与体内病毒发病机制之间的联系。

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Establishment of a system to quantify wild-type herpes simplex virus–induced cell–cell fusion reveals a role of N-glycosylation of HSV-1 envelope glycoprotein B in cell–cell fusion

Wild-type herpes simplex virus (HSV) strains infrequently mediate cell–cell fusion in cell cultures and barely induce large multinucleated cells. In this study, we established a system to quantify infrequent cell–cell fusion induced by wild-type HSV strains. The established system clarified that the HSV-1 envelope glycoprotein B and its N-glycosylation at asparagine at position 141 were required for efficient cell–cell fusion. This study provides a link between cell–cell fusion induced by wild-type HSV-1 and viral pathogenesis in vivo.

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.