抗TNFα治疗后轴性脊柱炎患者的单细胞分析:实验数据和文献综述。

IF 8.4 2区 医学 Q1 ALLERGY Clinical Reviews in Allergy & Immunology Pub Date : 2023-08-01 Epub Date: 2023-03-08 DOI:10.1007/s12016-023-08959-z
Zhi-Bin Zhao, Zhen-Hua Bian, Zhang-Mei Lin, Shu-Fan Wu, Jie Long, Yang Cui, Yang Li, Wende Li, Er-Wei Sun, Zhe-Xiong Lian, Yi He
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引用次数: 0

摘要

轴性脊椎关节炎(Ax-SpA)是一种主要影响轴性关节的慢性炎症性疾病,最常见于年轻男性。然而,参与Ax-SpA的确切免疫细胞亚群仍不清楚。我们的研究使用单细胞转录组学和蛋白质组学测序对Ax-SpA患者在抗TNFα治疗前后的外周免疫状况进行了表征,并阐明了抗TNFα在单细胞水平上的治疗效果。首先,我们发现Ax-SpA患者的外周粒细胞和单核细胞显著增加。其次,我们发现了一种功能更强的调节性T细胞亚型,它存在于滑膜液中,并在患者治疗后增加。第三,我们发现了一簇具有更强炎症和趋化特性的炎症单核细胞亚群。观察到经典单核细胞和粒细胞之间通过CXCL8/2-CXCR1/2信号通路的潜在相互作用,治疗后这种相互作用减少。总之,这些结果定义了Ax-SpA患者在抗TNFα治疗前后的复杂表达谱,并促进了我们对Ax-SpA病人免疫图谱的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Single-Cell Analysis of Patients with Axial Spondyloarthritis After Anti-TNFα Treatment: Experimental Data and Review of the Literature.

Axial spondyloarthritis (Ax-SpA) is a chronic inflammatory disease that predominantly affects the axial joints and is most common in young men. However, the precise immune cell subset involved in Ax-SpA remains unclear. Our study characterized the periphery immune landscape of Ax-SpA patients before and after anti-TNFα treatment using single-cell transcriptomics and proteomics sequencing and elucidated the effects of anti-TNFα treatment at the single-cell level. First, we found that peripheral granulocytes and monocytes significantly increased in Ax-SpA patients. Second, we identified a more functional subtype of regulatory T cells, which was present in synovial fluid and increased in patients after treatment. Third, we identified a cluster of inflammatory monocyte subset with stronger inflammatory and chemotactic characteristics. A potential interaction between classical monocytes and granulocytes via the CXCL8/2-CXCR1/2 signaling pathway was observed, which decreased after treatment. Together, these results defined the complex expression profiles and advanced our understanding of the immune atlas in Ax-SpA patients before and after anti-TNFα treatment.

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来源期刊
CiteScore
22.30
自引率
1.10%
发文量
58
审稿时长
6-12 weeks
期刊介绍: Clinical Reviews in Allergy & Immunology is a scholarly journal that focuses on the advancement of clinical management in allergic and immunologic diseases. The journal publishes both scholarly reviews and experimental papers that address the current state of managing these diseases, placing new data into perspective. Each issue of the journal is dedicated to a specific theme of critical importance to allergists and immunologists, aiming to provide a comprehensive understanding of the subject matter for a wide readership. The journal is particularly helpful in explaining how novel data impacts clinical management, along with advancements such as standardized protocols for allergy skin testing and challenge procedures, as well as improved understanding of cell biology. Ultimately, the journal aims to contribute to the improvement of care and management for patients with immune-mediated diseases.
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