慢性精神分裂症血清中多种形式单胺氧化酶的表征。

S A Al-Mudhaffar, N M Fahad
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引用次数: 0

摘要

半小时或一小时的孵育时间足以获得恒定的苯甲醛产量,并与酶的添加量成正比。每种形式的最佳pH值取决于所使用的底物浓度和所使用的缓冲液。这些形式被证明被高底物浓度抑制,形成无活性的酶-胺复合物,而丁胺和辛胺被发现是竞争性抑制剂。
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Characterization of the multiple forms of monoamine oxidase from chronic schizophrenic sera.

Either half or one hour incubation time was enough to get a constant production of benzylaldehyde and were proportional to the amount of enzyme added. The optimal temperature of MAO, I, II, III, IV are 60 degrees, 37 degrees, 60 degrees, 45 degrees, and 37 degrees C respectively, and they follow Arrhenius equation until these optimal temperatures. Each form have optimal pH depends on substrate concentration used and the buffer used. These forms were shown to be inhibited by high substrate concentration with formation of inactive enzyme-amine complex, whereas butyl- and octylamine was found to be competitive inhibitors. Isoniazid inhibit MAO II, III, IV and V forms in a non competitive fashion, whereas MAO I inhibited competitively with respect to the substrate. Semicarbazid inhibit MAO I. III, IV and V forms in a non competitive fashion, whereas MAO II inhibited competitively with respect to the substrate.

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