食品中霉菌定量检测方法比较。1 .交流:直接显微镜下霉菌计数的选择性染色程序(作者翻译)。

P Blaser
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引用次数: 0

摘要

提出的最重要的直接显微镜检测霉菌的方法在9种不同的非流体食品中进行了严格的比较,其中7种是自然成型的,2种是人工成型的。——对于非流质食物,无论是腐块试验、霍华德霉菌计数法还是Mossel(1975)提出的NaOH处理方法都不能令人满意。通过对真菌元素进行选择性产品特异性染色,可以显著改善菌种涂片中的直接显微检测。对于低蛋白食品,改良的钢琴染色法和低多糖食品,Perjod-Schiff反应被证明是合适的,并且易于进行。选择性染色菌种涂片的载玻片也允许高倍显微镜检查,因此真菌孢子和菌丝的区分是可能的。——真菌孢子、酵母细胞和菌丝片段可以用Seck(1976)的方法进行计数。菌丝片段也可根据霍华德霉菌计数法的规定进行记录。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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[Comparison of methods for the quantitative detection of moulds in foods. I. Communication: selective staining procedures for the directmicroscopic mould count (author's transl)].

The most important proposed direct microscopic method for the detection of moulds were critically compared in 9 different nonfluid foods, of which 7 were naturally and 2 were artifically moulded.--Neither the Rot fragment test, nor the Howard Mould count or the NaOH treatment method proposed by Mossel (1975) were satisfying with non-fluid foods.--A significant improvement of the direct microscopic detection in the Breed-smear could be achieved by selective product specific staining of the fungi elements.--For low protein foods, a modified Pianese staining, and for low polysaccharide foods the Perjod-Schiff reaction proved to be appropriate and easy to carry out.--The slides of the selectively stained Breed-smears also allow a microscopic examination with high magnifications, so that a differenciation of fungus spores and hyphaes is possible.--Fungus spores, yeast cells and hyphal fragments can be counted by the method of Seck (1976). Hyphal fragments can also be recorded according to the prescription of the Howard Mould Count method.

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